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Literature DB >> 12799443

The Upf-dependent decay of wild-type PPR1 mRNA depends on its 5'-UTR and first 92 ORF nucleotides.

B Kebaara¹, T Nazarenus, R Taylor, A Forch, A L Atkin.

Abstract

mRNAs containing premature translation termination codons (nonsense mRNAs) are targeted for deadenylation-independent degradation in a mechanism that depends on Upf1p, Upf2p and Upf3p. This decay pathway is often called nonsense- mediated mRNA decay (NMD). Nonsense mRNAs are decapped by Dcp1p and then degraded 5' to 3' by Xrn1p. In the yeast Saccharomyces cerevisiae, a significant number of wild-type mRNAs accumulate in upf mutants. Wild-type PPR1 mRNA is one of these mRNAs. Here we show that PPR1 mRNA degradation depends on the Upf proteins, Dcp1p, Xrn1p and Hrp1p. We have mapped an Upf1p-dependent destabilizing element to a region located within the 5'-UTR and the first 92 bases of the PPR1 ORF. This element targets PPR1 mRNA for Upf-dependent decay by a novel mechanism.

Entities: Gene Species

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Year: 2003 PMID： 12799443 PMCID： PMC162334 DOI： 10.1093/nar/gkg430

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

48 in total

1. Utilizing the GCN4 leader region to investigate the role of the sequence determinants in nonsense-mediated mRNA decay.

Authors: M J Ruiz-Echevarria; S W Peltz
Journal: EMBO J Date: 1996-06-03 Impact factor: 11.598

2. SMG-2 is a phosphorylated protein required for mRNA surveillance in Caenorhabditis elegans and related to Upf1p of yeast.

Authors: M F Page; B Carr; K R Anders; A Grimson; P Anderson
Journal: Mol Cell Biol Date: 1999-09 Impact factor: 4.272

The Upf-dependent decay of wild-type PPR1 mRNA depends on its 5'-UTR and first 92 ORF nucleotides.

1. Utilizing the GCN4 leader region to investigate the role of the sequence determinants in nonsense-mediated mRNA decay.

2. SMG-2 is a phosphorylated protein required for mRNA surveillance in Caenorhabditis elegans and related to Upf1p of yeast.

3. smg-7 is required for mRNA surveillance in Caenorhabditis elegans.

4. An internal open reading frame triggers nonsense-mediated decay of the yeast SPT10 mRNA.

5. Yeast Upf proteins required for RNA surveillance affect global expression of the yeast transcriptome.

6. An essential component of the decapping enzyme required for normal rates of mRNA turnover.

7. Identification of an additional gene required for eukaryotic nonsense mRNA turnover.

8. The majority of yeast UPF1 co-localizes with polyribosomes in the cytoplasm.

9. Mammalian orthologues of a yeast regulator of nonsense transcript stability.

10. Genomic detection of new yeast pre-mRNA 3'-end-processing signals.

1. Mating pheromone in Cryptococcus neoformans is regulated by a transcriptional/degradative "futile" cycle.

2. Regulatory sequence responsible for insulin destabilization of cytochrome P450 2B1 (CYP2B1) mRNA.

3. Copper tolerance of Saccharomyces cerevisiae nonsense-mediated mRNA decay mutants.

4. Gene set coregulated by the Saccharomyces cerevisiae nonsense-mediated mRNA decay pathway.

5. hUPF2 silencing identifies physiologic substrates of mammalian nonsense-mediated mRNA decay.

6. Role for Upf2p phosphorylation in Saccharomyces cerevisiae nonsense-mediated mRNA decay.

7. The alternative noncoding exons 1 of aromatase (Cyp19) gene modulate gene expression in a posttranscriptional manner.

8. Candida albicans Tup1 is involved in farnesol-mediated inhibition of filamentous-growth induction.

9. The 5' UTR negatively regulates quantitative and spatial expression from the ABI3 promoter.

10. UPF1, a conserved nonsense-mediated mRNA decay factor, regulates cyst wall protein transcripts in Giardia lamblia.