Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Fluorescent method for the detection of excreted ribonuclease around bacterial colonies.

Literature DB >> 4958882

Fluorescent method for the detection of excreted ribonuclease around bacterial colonies.

Abstract

Lanyi, Janos K. (Stanford University School of Medicine, Palo Alto, Calif.), and Joshua Lederberg. Fluorescent method for the detection of excreted ribonuclease around bacterial colonies. J. Bacteriol. 92:1469-1472. 1966.-A test for the release of extracellular ribonuclease by Bacillus subtilis colonles was developed. The method consists of incorporating acridine orange and ribonucleic acid into nutrient agar plates and viewing the grown bacterial colonies under ultraviolet light. Regions of ribonuclease secretion appear as dark halos around the colonies on a green fluorescent background. The theoretical basis and the utility of this test are discussed.

Entities: Chemical Species

Mesh：

Substances：

Year: 1966 PMID： 4958882 PMCID： PMC276447 DOI： 10.1128/jb.92.5.1469-1472.1966

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

12 in total

Fluorescent method for the detection of excreted ribonuclease around bacterial colonies.

1. ACRIDINE ORANGE AND ACRIFLAVIN INHIBIT DEOXYRIBONUCLEASE ACTION.

2. STUDIES ON THE NUCLEASES OF A STRAIN OF BACILLUS SUBTILIS.

3. The structure of the DNA-acridine complex.

4. Rapid method for determining the activity of microorganisms on nucleic acids.

5. Extracellular Deoxyribonucleases in Members of the Family Enterobacteriaceae.

6. AGGREGATION OF DYES BOUND TO POLYANIONS.

7. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE.

8. A diffusion plate technique for the detection of polynucleotide depolymerase and phosphatase activity.

9. The differentiation of three distinct desoxyrlbonucleases of group A Streptococci.

10. Evaluation of extracellular deoxyribonuclease activity in Pseudomonas.

1. Four hour-test for the identification of Enterobacteriaceae.

2. The involvement of extracellular enzymes in the metabolism of Bdellovibrio.

Review 3. Sporulation and the production of antibiotics, exoenzymes, and exotonins.

4. Criteria for categorizing early biochemical events occurring during sporulation of Bacillus subtilis.

5. Isolation of mutants of Staphylococcus aureus lacking extracellular nuclease activity.

6. Detection of nuclease activity in semisolid and broth cultures.

7. Isolation and genetic analysis of nuclease halo (nuh) mutants of Neurospora crassa.