Literature DB >> 1377648

Stereospecific assignments and chi 1 rotamers for FKBP when bound to ascomycin from 3JH alpha,H beta and 3HN,H beta coupling constants.

R X Xu1, E T Olejniczak, S W Fesik.   

Abstract

3JH alpha,H beta and 3JN,H beta coupling constants were measured for isotopically labeled FKBP when bound to the immunosuppressant, ascomycin, using a 1H-coupled 3D HCCH-TOCSY and 15N-coupled 3D HSQC-TOCSY experiment, respectively. From an analysis of these two sets of coupling constants, stereospecific beta-proton assignments and chi 1 rotamers for FKBP have been obtained. All of the chi 1 rotamers were consistent with the chi 1 angles measured in the X-ray crystal structure of the FKBP/FK506 complex, suggesting that the structures of the two complexes are similar.

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Year:  1992        PMID： 1377648     DOI： 10.1016/0014-5793(92)80881-g

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  8 in total

1.  On the calculation of ³Jαβ-coupling constants for side chains in proteins.

Authors:  Denise Steiner; Jane R Allison; Andreas P Eichenberger; Wilfred F van Gunsteren
Journal:  J Biomol NMR       Date:  2012-06-20       Impact factor: 2.835

2.  Measurement of (15)N- (1)H coupling constants in uniformly (15)N-labeled proteins: Application to the photoactive yellow protein.

Authors:  P Düx; B Whitehead; R Boelens; R Kaptein; G W Vuister
Journal:  J Biomol NMR       Date:  1997-10       Impact factor: 2.835

3.  NMR structure-based drug design.

Authors:  S W Fesik
Journal:  J Biomol NMR       Date:  1993-05       Impact factor: 2.835

4.  Stereospecific assignments of glycine in proteins by stereospecific deuteration and 15N labeling.

Authors:  R W Curley; M J Panigot; A P Hansen; S W Fesik
Journal:  J Biomol NMR       Date:  1994-05       Impact factor: 2.835

5.  Transforming between discrete and continuous angle distribution models: application to protein χ₁ torsions.

Authors:  Jürgen M Schmidt
Journal:  J Biomol NMR       Date:  2012-07-31       Impact factor: 2.835

6.  A J-modulated protonless NMR experiment characterizes the conformational ensemble of the intrinsically disordered protein WIP.

Authors:  Eva Rozentur-Shkop; Gil Goobes; Jordan H Chill
Journal:  J Biomol NMR       Date:  2016-11-14       Impact factor: 2.835

7.  Measurement of two- and three-bond 13C-1H J couplings to the C delta carbons of leucine residues in staphylococcal nuclease.

Authors:  G W Vuister; T Yamazaki; D A Torchia; A Bax
Journal:  J Biomol NMR       Date:  1993-05       Impact factor: 2.835

Review 8.  Probing Protein-Protein Interactions Using Asymmetric Labeling and Carbonyl-Carbon Selective Heteronuclear NMR Spectroscopy.

Authors:  Erik K Larsen; Cristina Olivieri; Caitlin Walker; Manu V S; Jiali Gao; David A Bernlohr; Marco Tonelli; John L Markley; Gianluigi Veglia
Journal:  Molecules       Date:  2018-08-03       Impact factor: 4.411
  8 in total

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