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Literature DB >> 1371846

Construction of a umuDC operon substitution mutation in Escherichia coli.

Abstract

Using a specialized transducing lambda phage, the umuDC operon of Escherichia coli was deleted and replaced with the chloramphenicol acetyltransferase gene. The delta (umuDC)595::cat mutation was subsequently transferred by generalized P1 transduction into a variety of genetic backgrounds. It is concluded that the UmuDC proteins, which are normally required for inducible mutagenesis, are not essential for cell survival.

Entities: Gene Species

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Year: 1992 PMID： 1371846 DOI： 10.1016/0165-7992(92)90012-7

Source DB: PubMed Journal: Mutat Res ISSN： 0027-5107 Impact factor: 2.433

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Cited

56 in total

1. A phenotype for enigmatic DNA polymerase II: a pivotal role for pol II in replication restart in UV-irradiated Escherichia coli.

Authors: S Rangarajan; R Woodgate; M F Goodman
Journal: Proc Natl Acad Sci U S A Date: 1999-08-03 Impact factor: 11.205

2. Intrinsic polymerase activities of UmuD'(2)C and MucA'(2)B are responsible for their different mutagenic properties during bypass of a T-T cis-syn cyclobutane dimer.

Authors: P I O'Grady; A Borden; D Vandewiele; A Ozgenc; R Woodgate; C W Lawrence
Journal: J Bacteriol Date: 2000-04 Impact factor: 3.490

3. The SOS response regulates adaptive mutation.

Authors: G J McKenzie; R S Harris; P L Lee; S M Rosenberg
Journal: Proc Natl Acad Sci U S A Date: 2000-06-06 Impact factor: 11.205

4. Escherichia coli DNA polymerase III can replicate efficiently past a T-T cis-syn cyclobutane dimer if DNA polymerase V and the 3' to 5' exonuclease proofreading function encoded by dnaQ are inactivated.

Authors: Angela Borden; Paul I O'Grady; Dominique Vandewiele; Antonio R Fernández de Henestrosa; Christopher W Lawrence; Roger Woodgate
Journal: J Bacteriol Date: 2002-05 Impact factor: 3.490

10. Nucleotide excision repair or polymerase V-mediated lesion bypass can act to restore UV-arrested replication forks in Escherichia coli.

Authors: Charmain T Courcelle; Jerilyn J Belle; Justin Courcelle
Journal: J Bacteriol Date: 2005-10 Impact factor: 3.490

Construction of a umuDC operon substitution mutation in Escherichia coli.

1. A phenotype for enigmatic DNA polymerase II: a pivotal role for pol II in replication restart in UV-irradiated Escherichia coli.

2. Intrinsic polymerase activities of UmuD'(2)C and MucA'(2)B are responsible for their different mutagenic properties during bypass of a T-T cis-syn cyclobutane dimer.

3. The SOS response regulates adaptive mutation.

4. Escherichia coli DNA polymerase III can replicate efficiently past a T-T cis-syn cyclobutane dimer if DNA polymerase V and the 3' to 5' exonuclease proofreading function encoded by dnaQ are inactivated.

5. The miaA mutator phenotype of Escherichia coli K-12 requires recombination functions.

6. Escherichia coli umuDC mutants: DNA sequence alterations and UmuD cleavage.

7. Lack of strand bias in UV-induced mutagenesis in Escherichia coli.

8. The Escherichia coli dnaN159 mutant displays altered DNA polymerase usage and chronic SOS induction.

Review 9. A new model for SOS-induced mutagenesis: how RecA protein activates DNA polymerase V.

10. Nucleotide excision repair or polymerase V-mediated lesion bypass can act to restore UV-arrested replication forks in Escherichia coli.