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Literature DB >> 1371250

CpG methylated minichromosomes become inaccessible for V(D)J recombination after undergoing replication.

Abstract

The physical parameters controlling the accessibility of antigen receptor loci to the V(D)J recombination activity are unknown. We have used minichromosome substrates to study the role that CpG methylation might play in controlling V(D)J recombination site accessibility. We find that CpG methylation decreases the V(D)J recombination of these substrates more than 100-fold. The decrease correlates with a considerable increase in resistance to endonuclease digestion of the methylated minichromosome DNA. The minichromosomes acquire resistance to both the intracellular V(D)J recombinase and exogenous endonuclease only after DNA replication. Therefore, CpG methylation specifies a chromatin structure that, upon DNA replication, is resistant to eukaryotic site-specific recombination. These findings are important to V(D)J recombination as well as to the chromatin assembly of methylated DNA during replication.

Entities: Chemical

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Year: 1992 PMID： 1371250 PMCID： PMC556452 DOI： 10.1002/j.1460-2075.1992.tb05054.x

Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598

50 in total

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Authors: R R Meehan; J D Lewis; S McKay; E L Kleiner; A P Bird
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3. Effect of in vitro DNA methylation on beta-globin gene expression.

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4. Mechanism of action of rat liver DNA methylase. I. Interaction with double-stranded methyl-acceptor DNA.

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Review 5. Development of the primary antibody repertoire.

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6. Kanamycin-resistant vectors that are analogues of plasmids pUC8, pUC9, pEMBL8 and pEMBL9.

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7. DNase I sensitivity of immunoglobulin light chain genes in Abelson murine leukemia virus transformed pre-B cell lines.

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Journal: Nucleic Acids Res Date: 1989-07-11 Impact factor: 16.971

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9. Evolutionary comparison of the avian IgL locus: combinatorial diversity plays a role in the generation of the antibody repertoire in some avian species.

Authors: W T McCormack; L M Carlson; L W Tjoelker; C B Thompson
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Authors: Y Ichihara; H Matsuoka; Y Kurosawa
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49 in total

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Authors: S R Cherry; D Baltimore
Journal: Proc Natl Acad Sci U S A Date: 1999-09-14 Impact factor: 11.205

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Authors: S R Cherry; C Beard; R Jaenisch; D Baltimore
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Journal: EMBO J Date: 2002-10-01 Impact factor: 11.598

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Authors: Yanhong Ji; Jianmin Zhang; Alfred Ian Lee; Howard Cedar; Yehudit Bergman
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6. Characterization of MeCP2, a vertebrate DNA binding protein with affinity for methylated DNA.

Authors: R R Meehan; J D Lewis; A P Bird
Journal: Nucleic Acids Res Date: 1992-10-11 Impact factor: 16.971

7. DNA methylation has a local effect on transcription and histone acetylation.

Authors: Ryan A Irvine; Iping G Lin; Chih-Lin Hsieh
Journal: Mol Cell Biol Date: 2002-10 Impact factor: 4.272

Review 8. Allelic exclusion of immunoglobulin genes: models and mechanisms.

Authors: Christian Vettermann; Mark S Schlissel
Journal: Immunol Rev Date: 2010-09 Impact factor: 12.988

9. Structural adaptations in the interaction of EcoRI endonuclease with methylated GAATTC sites.

Authors: L Jen-Jacobson; L E Engler; D R Lesser; M R Kurpiewski; C Yee; B McVerry
Journal: EMBO J Date: 1996-06-03 Impact factor: 11.598

10. Type 2 NF1 deletions are highly unusual by virtue of the absence of nonallelic homologous recombination hotspots and an apparent preference for female mitotic recombination.

Authors: Katharina Steinmann; David N Cooper; Lan Kluwe; Nadia A Chuzhanova; Cornelia Senger; Eduard Serra; Conxi Lazaro; Montserrat Gilaberte; Katharina Wimmer; Viktor-Felix Mautner; Hildegard Kehrer-Sawatzki
Journal: Am J Hum Genet Date: 2007-10-31 Impact factor: 11.025