Lutz Claes1, Kerstin Eckert-Hübner, Peter Augat. 1. Institute for Orthopaedic Research and Biomechanics, University of Ulm, Helmholtzstrasse 14, 89081 Ulm, Germany. lutz.claes@medizin.uni-ulm.de
Abstract
BACKGROUND: Revascularization of a fracture depends on fracture stability and fracture gap conditions. The aim of the study was to determine quantitatively the revascularization and tissue differentiation in an animal model with different fracture gaps and controlled biomechanical conditions. MATERIALS AND METHOD: The study was performed on ten sheep with an osteotomy on the right metatarsal. The fracture was stabilized by an external fixator that allowed adjustable axial interfragmentary movement. Two groups of five sheep each were adjusted to a medium sized gap (M, 2.1 mm) and a large gap (L, 5.7 mm) under comparable interfragmentary strain (30-32%). The animals were killed after 9 weeks, and the metatarsals were prepared for undecalcified histology and analysis of tissue differentiation and vessel distribution. RESULTS: Group M showed significantly more revascularization (M=1.62, L=0.85 vessels/mm2), more bone formation (M=37.2%, L=13.9%) and less fibrocartilage tissue (M=18.1%, L=39.1%) than group L. Larger vessels (>40 microm) were found mainly in the medullary channel, and smaller vessels (<20 microm) mainly in the peripheral callus. Histologically, group M showed partial bony bridging of the osteotomy gap, and the group L had delayed healing. CONCLUSION: A good reduction of a fracture with small interfragmentary gaps is important for its revascularization and healing.
BACKGROUND: Revascularization of a fracture depends on fracture stability and fracture gap conditions. The aim of the study was to determine quantitatively the revascularization and tissue differentiation in an animal model with different fracture gaps and controlled biomechanical conditions. MATERIALS AND METHOD: The study was performed on ten sheep with an osteotomy on the right metatarsal. The fracture was stabilized by an external fixator that allowed adjustable axial interfragmentary movement. Two groups of five sheep each were adjusted to a medium sized gap (M, 2.1 mm) and a large gap (L, 5.7 mm) under comparable interfragmentary strain (30-32%). The animals were killed after 9 weeks, and the metatarsals were prepared for undecalcified histology and analysis of tissue differentiation and vessel distribution. RESULTS: Group M showed significantly more revascularization (M=1.62, L=0.85 vessels/mm2), more bone formation (M=37.2%, L=13.9%) and less fibrocartilage tissue (M=18.1%, L=39.1%) than group L. Larger vessels (>40 microm) were found mainly in the medullary channel, and smaller vessels (<20 microm) mainly in the peripheral callus. Histologically, group M showed partial bony bridging of the osteotomy gap, and the group L had delayed healing. CONCLUSION: A good reduction of a fracture with small interfragmentary gaps is important for its revascularization and healing.
Authors: Michael J Gardner; Sara M Putnam; Ambrose Wong; Philipp N Streubel; Akhilesh Kotiya; Matthew J Silva Journal: J Orthop Sci Date: 2011-03-31 Impact factor: 1.805
Authors: Christian W Müller; Ronny Pfeifer; Karen Meier; Sebastian Decker; Janin Reifenrath; Thomas Gösling; Volker Wesling; Christian Krettek; Christof Hurschler; Manuel Krämer Journal: Biomed Res Int Date: 2015-06-08 Impact factor: 3.411