Literature DB >> 1367625

Xylitol production by recombinant Saccharomyces cerevisiae.

J Hallborn1, M Walfridsson, U Airaksinen, H Ojamo, B Hahn-Hägerdal, M Penttilä, S Keräsnen.   

Abstract

We obtained efficient conversion of xylose to xylitol by transforming Saccharomyces cerevisiae with the gene encoding the xylose reductase (XR) of Pichia stipitis CBS 6054. Comparison of the chromosomal and cDNA copies of the XYL1 gene showed that the genomic XYL1 contains no introns, and an XR monomer of 318 amino acids (35,985 D) is encoded by an open reading frame of 954 bp. The amino acid sequence of the P. stipitis XR is similar to several aldose reductases, suggesting that P. stipitis XR is part of the aldoketo reductase superfamily. S. cerevisiae transformed with the XYL1 gene gave over 95% conversion of xylose into xylitol, a yield not obtainable with natural xylose utilizing yeasts.

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Year:  1991        PMID： 1367625     DOI： 10.1038/nbt1191-1090

Source DB:  PubMed          Journal:  Biotechnology (N Y)        ISSN： 0733-222X


  37 in total

1.  Production of xylitol from D-xylose by a xylitol dehydrogenase gene-disrupted mutant of Candida tropicalis.

Authors:  Byoung Sam Ko; Jinmi Kim; Jung Hoe Kim
Journal:  Appl Environ Microbiol       Date:  2006-06       Impact factor: 4.792

Review 2.  Metabolic engineering of Saccharomyces cerevisiae.

Authors:  S Ostergaard; L Olsson; J Nielsen
Journal:  Microbiol Mol Biol Rev       Date:  2000-03       Impact factor: 11.056

3.  A glycerol-3-phosphate dehydrogenase-deficient mutant of Saccharomyces cerevisiae expressing the heterologous XYL1 gene.

Authors:  G Lidén; M Walfridsson; R Ansell; M Anderlund; L Adler; B Hahn-Hägerdal
Journal:  Appl Environ Microbiol       Date:  1996-10       Impact factor: 4.792

4.  Investigation of the role of a conserved glycine motif in the Saccharomyces cerevisiae xylose reductase.

Authors:  Byron C H Chu; Hung Lee
Journal:  Curr Microbiol       Date:  2006-06-26       Impact factor: 2.188

5.  NAD(P)H-dependent aldose reductase from the xylose-assimilating yeast Candida tenuis. Isolation, characterization and biochemical properties of the enzyme.

Authors:  W Neuhauser; D Haltrich; K D Kulbe; B Nidetzky
Journal:  Biochem J       Date:  1997-09-15       Impact factor: 3.857

6.  Effects of environmental conditions on production of xylitol byCandida boidinii.

Authors:  E Vandeska; S Amartey; S Kuzmanova; T Jeffries
Journal:  World J Microbiol Biotechnol       Date:  1995-03       Impact factor: 3.312

7.  Influence of cosubstrate concentration on xylose conversion by recombinant, XYL1-expressing Saccharomyces cerevisiae: a comparison of different sugars and ethanol as cosubstrates.

Authors:  N Q Meinander; B Hahn-Hägerdal
Journal:  Appl Environ Microbiol       Date:  1997-05       Impact factor: 4.792

8.  Xylitol formation and reduction equivalent generation during anaerobic xylose conversion with glucose as cosubstrate in recombinant Saccharomyces cerevisiae expressing the xyl1 gene.

Authors:  H N Thestrup; B Hahn-Hägerdal
Journal:  Appl Environ Microbiol       Date:  1995-05       Impact factor: 4.792

9.  Bulk segregant analysis by high-throughput sequencing reveals a novel xylose utilization gene from Saccharomyces cerevisiae.

Authors:  Jared W Wenger; Katja Schwartz; Gavin Sherlock
Journal:  PLoS Genet       Date:  2010-05-13       Impact factor: 5.917

10.  Structural and functional properties of a yeast xylitol dehydrogenase, a Zn2+-containing metalloenzyme similar to medium-chain sorbitol dehydrogenases.

Authors:  R Lunzer; Y Mamnun; D Haltrich; K D Kulbe; B Nidetzky
Journal:  Biochem J       Date:  1998-11-15       Impact factor: 3.857
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