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Literature DB >> 1367534

Production of pectin methylesterase from Erwinia chrysanthemi B374 in Bacillus subtilis.

R Heikinheimo¹, H Hemilä, R Pakkanen, I Palva.

Abstract

The gene coding for pectin methylesterase (PME) of Erwinia chrysanthemi B374 (pme) was cloned by a polymerase chain reaction. The pme gene was expressed in Bacillus subtilis using a secretion vector based on the promoter and signal sequence of the alpha-amylase gene from B. amyloliquefaciens. The cultivation of B. subtilis cells carrying the cloned pme resulted in efficient secretion of PME into the culture medium based on enzymatic and sodium dodecyl sulphate-poly-acrylamide gel electrophoresis characterizations. The NH2-terminal sequence analysis of the secreted PME revealed two different NH2-termini. Heterologous processing was probably due to a second putative signal peptidase cleavage site at the joint region between the PME and alpha-amylase signal peptide.

Entities: Chemical Species

Mesh：

Substances：

Year: 1991 PMID： 1367534 DOI： 10.1007/bf00180635

Source DB: PubMed Journal: Appl Microbiol Biotechnol ISSN： 0175-7598 Impact factor: 4.813

11 in total

Review 1. Pectic enzymes.

Authors: L Rexová-Benková; O Markovic
Journal: Adv Carbohydr Chem Biochem Date: 1976 Impact factor: 12.200

2. Production and secretion of pertussis toxin subunits in Bacillus subtilis.

Authors: P Saris; S Taira; U Airaksinen; A Palva; M Sarvas; I Palva; K Runeberg-Nyman
Journal: FEMS Microbiol Lett Date: 1990-03-01 Impact factor: 2.742

3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

4. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.

Authors: D A Melton; P A Krieg; M R Rebagliati; T Maniatis; K Zinn; M R Green
Journal: Nucleic Acids Res Date: 1984-09-25 Impact factor: 16.971

3. Production of heterologous proteins in Bacillus subtilis: the effect of the joint between signal sequence and mature protein on yield.

Authors: H Hemilä; M Sibakov
Journal: Appl Microbiol Biotechnol Date: 1991-10 Impact factor: 4.813

3 in total

Production of pectin methylesterase from Erwinia chrysanthemi B374 in Bacillus subtilis.

Review 1. Pectic enzymes.

2. Production and secretion of pertussis toxin subunits in Bacillus subtilis.

3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

4. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.

5. Secretory S complex of Bacillus subtilis: sequence analysis and identity to pyruvate dehydrogenase.

6. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis.

7. DNA sequencing with chain-terminating inhibitors.

8. Molecular cloning of alpha-amylase gene from Bacillus amyloliquefaciens and its expression in B. subtilis.

9. Molecular cloning and nucleotide sequence of the pectin methyl esterase gene of Erwinia chrysanthemi B374.

10. Studies on transformation of Escherichia coli with plasmids.

Review 1. Protein secretion in Bacillus species.

2. Molecular Cloning, Expression and Characterization of Pectin Methylesterase (CtPME) from Clostridium thermocellum.

3. Production of heterologous proteins in Bacillus subtilis: the effect of the joint between signal sequence and mature protein on yield.