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Literature DB >> 2837615

Molecular cloning and nucleotide sequence of the pectin methyl esterase gene of Erwinia chrysanthemi B374.

Abstract

The gene encoding pectin methyl esterase (pme) has been cloned from Erwinia chrysanthemi B374. Expression of pme in Escherichia coli allowed the enzyme to be characterized. Pectin methyl esterase (PME) was found to have an apparent molecular weight of 36,000 Daltons and an isoelectric point of approximately 9.9. The structural gene was sequenced and consists of a 1098-bp open reading frame encoding a polypeptide of 39,318 Daltons, which includes an amino-terminal signal peptide. The isolation of the Erwinia gene provides a simple method for the production of PME free from depolymerizing pectinases thereby extending its potential uses.

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Year: 1988 PMID： 2837615 DOI： 10.1111/j.1365-2958.1988.tb00026.x

Source DB: PubMed Journal: Mol Microbiol ISSN： 0950-382X Impact factor: 3.501

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Cited

17 in total

1. Characterization and functional expression of a ubiquitously expressed tomato pectin methylesterase.

Authors: J Gaffe; M E Tiznado; A K Handa
Journal: Plant Physiol Date: 1997-08 Impact factor: 8.340

2. Molecular and genetic characterization of two pollen-expressed genes that have sequence similarity to pectate lyases of the plant pathogen Erwinia.

Authors: R A Wing; J Yamaguchi; S K Larabell; V M Ursin; S McCormick
Journal: Plant Mol Biol Date: 1990-01 Impact factor: 4.076

8. Inactivation, sequence, and lacZ fusion analysis of a regulatory locus required for repression of nitrogen fixation genes in Rhodobacter capsulatus.

Authors: R G Kranz; V M Pace; I M Caldicott
Journal: J Bacteriol Date: 1990-01 Impact factor: 3.490

9. A gene showing sequence similarity to pectin esterase is specifically expressed in developing pollen of Brassica napus. Sequences in its 5' flanking region are conserved in other pollen-specific promoters.

Authors: D Albani; I Altosaar; P G Arnison; S F Fabijanski
Journal: Plant Mol Biol Date: 1991-04 Impact factor: 4.076

10. Disulfide bridges in tomato pectinesterase: variations from pectinesterases of other species; conservation of possible active site segments.

Authors: O Markovic; H Jörnvall
Journal: Protein Sci Date: 1992-10 Impact factor: 6.725

Molecular cloning and nucleotide sequence of the pectin methyl esterase gene of Erwinia chrysanthemi B374.

1. Characterization and functional expression of a ubiquitously expressed tomato pectin methylesterase.

2. Molecular and genetic characterization of two pollen-expressed genes that have sequence similarity to pectate lyases of the plant pathogen Erwinia.

3. Modification of tomato and Aspergillus niger pectinesterases with diethyl pyrocarbonate.

4. Regulation and role in pathogenicity of Erwinia chrysanthemi 3937 pectin methylesterase.

5. Characterization of a pollen-expressed gene encoding a putative pectin esterase of Petunia inflata.

6. Molecular Cloning, Expression and Characterization of Pectin Methylesterase (CtPME) from Clostridium thermocellum.

7. Endopolygalacturonase is not required for pathogenicity of Cochliobolus carbonum on maize.

8. Inactivation, sequence, and lacZ fusion analysis of a regulatory locus required for repression of nitrogen fixation genes in Rhodobacter capsulatus.

9. A gene showing sequence similarity to pectin esterase is specifically expressed in developing pollen of Brassica napus. Sequences in its 5' flanking region are conserved in other pollen-specific promoters.

10. Disulfide bridges in tomato pectinesterase: variations from pectinesterases of other species; conservation of possible active site segments.