Literature DB >> 1366661

The use of macroporous gelatin carriers for the cultivation of mammalian cells in fluidised bed reactors.

M Reiter1, O Hohenwarter, T Gaida, N Zach, C Schmatz, G Blüml, F Weigang, K Nilsson, H Katinger.   

Abstract

A fluidised bed system for the cultivation of mammalian cells on a new type of macroporous gelatin microcarrier is described. The volumetric cell densities achieved under controlled conditions for two 'standard cell lines' (VERO, CHO) were one order of magnitude higher compared to conventional techniques using spherical microcarriers. The system can be potentially used for both anchorage dependent and independent cells.

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Year:  1990        PMID： 1366661     DOI： 10.1007/bf00365491

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  6 in total

1.  Adhesion and spreading of cells on charged surfaces.

Authors:  N G Maroudas
Journal:  J Theor Biol       Date:  1975-02       Impact factor: 2.691

2.  Fixed bed porous glass sphere (porosphere) bioreactors for animal cells.

Authors:  D Looby; J B Griffiths
Journal:  Cytotechnology       Date:  1988-11       Impact factor: 2.058

3.  The production of foot-and-mouth disease virus from BHK 21 C 13 cells grown on the surface of glass spheres.

Authors:  R E Spier; J P Whiteside
Journal:  Biotechnol Bioeng       Date:  1976-05       Impact factor: 4.530

4.  Growth of cell-strains and primary cells on micro-carriers in homogeneous culture.

Authors:  A L van Wezel
Journal:  Nature       Date:  1967-10-07       Impact factor: 49.962

5.  Alternative surfaces for microcarrier culture of animal cells.

Authors:  C Gebb; J M Clark; M D Hirtenstein; G Lindgren; U Lindskog; B Lundgren; P Vretblad
Journal:  Dev Biol Stand       Date:  1981

6.  High-performance liquid chromatographic determination of metabolic products for fermentation control of mammalian cell culture: analysis of carbohydrates, organic acids and orthophosphate using refractive index and ultraviolet detectors.

Authors:  F Weigang; M Reiter; A Jungbauer; H Katinger
Journal:  J Chromatogr       Date:  1989-12-29
  6 in total
  7 in total

1.  Scale-up of suspension and anchorage-dependent animal cells.

Authors:  B Griffiths
Journal:  Mol Biotechnol       Date:  2001-03       Impact factor: 2.695

2.  Improved microscopic observation of mammalian cells on microcarriers by fluorescent staining.

Authors:  T J Nikolai; M V Peshwa; S Goetghebeur; W S Hu
Journal:  Cytotechnology       Date:  1991-02       Impact factor: 2.058

3.  Determination of division rates of rCHO cells in high density and immobilized fermentation systems by flow cytometry.

Authors:  N Borth; M Reiter; G Blüml; C Schmatz; T Gaida; H Katinger
Journal:  Cytotechnology       Date:  1992       Impact factor: 2.058

4.  Flow cytometry and two-dimensional electrophoresis (2-DE) for system evaluation of long term continuous perfused animal cell cultures in macroporous beads.

Authors:  M Reiter; N Borth; G Blüml; K Wimmer; H Harant; N Zach; T Gaida; C Schmatz; H Katinger
Journal:  Cytotechnology       Date:  1992       Impact factor: 2.058

5.  Cell growth and protein formation on various microcarriers.

Authors:  D Kong; M Chen; R Gentz; J Zhang
Journal:  Cytotechnology       Date:  1999-03       Impact factor: 2.058

6.  High cell density and productivity culture of Chinese hamster ovary cells in a fluidized bed bioreactor.

Authors:  D Kong; S Cardak; M Chen; R Gentz; J Zhang
Journal:  Cytotechnology       Date:  1999-05       Impact factor: 2.058

7.  Cultivation of anchorage-dependent animal cells in microsphere-induced aggregate culture.

Authors:  S Goetghebeur; W S Hu
Journal:  Appl Microbiol Biotechnol       Date:  1991-03       Impact factor: 4.813
  7 in total

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