Literature DB >> 1367155

Improved microscopic observation of mammalian cells on microcarriers by fluorescent staining.

T J Nikolai1, M V Peshwa, S Goetghebeur, W S Hu.   

Abstract

Many microcarriers used for the cultivation of animal cells do not allow for convenient microscopic observation of cell morphology and viability due to their optical properties. Using fluorescent viable stain combining fluorescein diacetate and ethidium bromide, we observed the distribution, morphology and viability of cells on various microcarriers.

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Year:  1991        PMID： 1367155     DOI： 10.1007/bf00365430

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  14 in total

1.  Factors affecting cell attachment, spreading, and growth on derivatized microcarriers. I. Establishment of working system and effect of the type of the amino-charged groups.

Authors:  S Reuveny; A Mizrahi; M Kotler; A Freeman
Journal:  Biotechnol Bioeng       Date:  1983-02       Impact factor: 4.530

2.  Growth of three established cell lines on glass microcarriers.

Authors:  J Varani; M Dame; T F Beals; J A Wass
Journal:  Biotechnol Bioeng       Date:  1983-05       Impact factor: 4.530

3.  Attachment and growth of mammalian cells on microcarriers with different ion exchange capacities.

Authors:  V B Himes; W S Hu
Journal:  Biotechnol Bioeng       Date:  1987-06       Impact factor: 4.530

4.  A rapid, quantitative fluorescence assay for cell damage by cytotoxic antibodies.

Authors:  M Edidin
Journal:  J Immunol       Date:  1970-05       Impact factor: 5.422

5.  The use of Cytodex 3 microcarriers and reduced-serum media for the production of nerve growth promoters from chicken heart cells.

Authors:  G Norrgren; T Ebendal; C Gebb; D Wikström
Journal:  Dev Biol Stand       Date:  1983

6.  A test of granulocyte membrane integrity and phagocytic function.

Authors:  F Dankberg; M D Persidsky
Journal:  Cryobiology       Date:  1976-08       Impact factor: 2.487

7.  Biosilon a new microcarrier.

Authors:  A Johansson; V Nielsen
Journal:  Dev Biol Stand       Date:  1980

8.  Cultivation of anchorage-dependent animal cells in microsphere-induced aggregate culture.

Authors:  S Goetghebeur; W S Hu
Journal:  Appl Microbiol Biotechnol       Date:  1991-03       Impact factor: 4.813

9.  Growth of anchorage dependent mammalian cells on glycine-derivatized polystyrene in suspension culture.

Authors:  M J Kuo; C Lewis; R A Martin; R E Miller; R A Schoenfeld; J M Schuck; B S Wildi
Journal:  In Vitro       Date:  1981-10

10.  Serial propagation of mammalian cells on microcarriers.

Authors:  W S Hu; D J Giard; D I Wang
Journal:  Biotechnol Bioeng       Date:  1985-10       Impact factor: 4.530
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  5 in total

1.  Changes in animal cell natural aggregates in suspended batch cultures.

Authors:  J L Moreira; P M Alves; J G Aunins; M J Carrondo
Journal:  Appl Microbiol Biotechnol       Date:  1994-04       Impact factor: 4.813

2.  High density culture of mammalian cells with dynamic perfusion based on on-line oxygen uptake rate measurements.

Authors:  Y S Kyung; M V Peshwa; D M Gryte; W S Hu
Journal:  Cytotechnology       Date:  1994       Impact factor: 2.058

3.  Cultivation of anchorage-dependent animal cells in microsphere-induced aggregate culture.

Authors:  S Goetghebeur; W S Hu
Journal:  Appl Microbiol Biotechnol       Date:  1991-03       Impact factor: 4.813

4.  Primary culture of rat hepatocytes entrapped in cylindrical collagen gels: an in vitro system with application to the bioartificial liver. Rat hepatocytes cultured in cylindrical collagen gels.

Authors:  S L Nyberg; R A Shatford; W D Payne; W S Hu; F B Cerra
Journal:  Cytotechnology       Date:  1992       Impact factor: 2.058

5.  Development of an optical system for the non-invasive tracking of stem cell growth on microcarriers.

Authors:  Akinlolu Oyekunle Oluseun Odeleye; Sara Castillo-Avila; Mathew Boon; Haydn Martin; Karen Coopman
Journal:  Biotechnol Bioeng       Date:  2017-05-23       Impact factor: 4.530
  5 in total

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