Literature DB >> 1355481

Properties of a HeLa cell 3' exonuclease specific for degrading poly(A) tails of mammalian mRNA.

J Aström1, A Aström, A Virtanen.   

Abstract

A HeLa cell 3'-exonuclease with properties of a mammalian mRNA poly(A) tail-removing enzyme has been characterized. The exonuclease shows high specificity for the poly(A) tail, and it is single strand-specific and requires a 3'-hydroxyl group for its activity. During degradation 5'-AMP is liberated as a product, and a 3'-OH group is left on the last adenosine residue of the remaining poly(A) tail. The activity is inhibited by 5'-AMP and can be competed by poly(A)-containing mRNA or poly(A). Based on these findings we propose a reaction pathway for poly(A) tail removal catalyzed by the HeLa cell poly(A) tail-specific 3' exonuclease.

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Year:  1992        PMID： 1355481

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  32 in total

1.  Characterization of deadenylation in trypanosome extracts and its inhibition by poly(A)-binding protein Pab1p.

Authors:  Joseph Milone; Jeffrey Wilusz; Vivian Bellofatto
Journal:  RNA       Date:  2004-03       Impact factor: 4.942

2.  AtHESPERIN: a novel regulator of circadian rhythms with poly(A)-degrading activity in plants.

Authors:  Costas Delis; Afrodite Krokida; Anastasia Tomatsidou; Daniela Tsikou; Rafailia A A Beta; Maria Tsioumpekou; Julietta Moustaka; Georgios Stravodimos; Demetres D Leonidas; Nikolaos A A Balatsos; Kalliope K Papadopoulou
Journal:  RNA Biol       Date:  2016       Impact factor: 4.652

3.  Poly(A)-specific ribonuclease mediates 3'-end trimming of Argonaute2-cleaved precursor microRNAs.

Authors:  Mayuko Yoda; Daniel Cifuentes; Natsuko Izumi; Yuriko Sakaguchi; Tsutomu Suzuki; Antonio J Giraldez; Yukihide Tomari
Journal:  Cell Rep       Date:  2013-10-24       Impact factor: 9.423

4.  PCF11 encodes a third protein component of yeast cleavage and polyadenylation factor I.

Authors:  N Amrani; M Minet; F Wyers; M E Dufour; L P Aggerbeck; F Lacroute
Journal:  Mol Cell Biol       Date:  1997-03       Impact factor: 4.272

5.  Control of mRNA stability by SAPKs.

Authors:  Miguel A Rodríguez-Gabriel; Paul Russell
Journal:  Top Curr Genet       Date:  2008-01-01

6.  The poly(A) tail inhibits the assembly of a 3'-to-5' exonuclease in an in vitro RNA stability system.

Authors:  L P Ford; P S Bagga; J Wilusz
Journal:  Mol Cell Biol       Date:  1997-01       Impact factor: 4.272

7.  Identification of mRNA decapping activities and an ARE-regulated 3' to 5' exonuclease activity in trypanosome extracts.

Authors:  Joseph Milone; Jeffrey Wilusz; Vivian Bellofatto
Journal:  Nucleic Acids Res       Date:  2002-09-15       Impact factor: 16.971

8.  AUUUA is not sufficient to promote poly(A) shortening and degradation of an mRNA: the functional sequence within AU-rich elements may be UUAUUUA(U/A)(U/A).

Authors:  C A Lagnado; C Y Brown; G J Goodall
Journal:  Mol Cell Biol       Date:  1994-12       Impact factor: 4.272

9.  PAN3 encodes a subunit of the Pab1p-dependent poly(A) nuclease in Saccharomyces cerevisiae.

Authors:  C E Brown; S Z Tarun; R Boeck; A B Sachs
Journal:  Mol Cell Biol       Date:  1996-10       Impact factor: 4.272

10.  Recognition of adenosine residues by the active site of poly(A)-specific ribonuclease.

Authors:  Niklas Henriksson; Per Nilsson; Mousheng Wu; Haiwei Song; Anders Virtanen
Journal:  J Biol Chem       Date:  2009-11-09       Impact factor: 5.157
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