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Literature DB >> 1339400

Alpha-1-antichymotrypsin variant detected by PCR-single strand conformation polymorphism (PCR-SSCP) and direct sequencing.

M Tsuda¹, Y Sei, M Matsumoto, H Kamiguchi, M Yamamoto, Y Shinohara, T Igarashi, M Yamamura.

Abstract

A new mutant alpha-1-antichymotrypsin (variant ACT) was found by polymerase chain reaction single strand conformation polymorphism and direct sequencing. In this variant ACT, two bases (AA) were deleted from codon 391. This resulted in a different amino acid sequence downstream of the deletion point, elongating the peptide chain by 10 amino acids.

Entities: Gene

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Year: 1992 PMID： 1339400 DOI： 10.1007/bf00220480

Source DB: PubMed Journal: Hum Genet ISSN： 0340-6717 Impact factor: 4.132

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References

9 in total

1. Detection of a new mutant alpha-1-antichymotrypsin in patients with occlusive-cerebrovascular disease.

Authors: M Tsuda; Y Sei; M Yamamura; M Yamamoto; Y Shinohara
Journal: FEBS Lett Date: 1992-06-08 Impact factor: 4.124

2. Cloning, expression, purification, and biological activity of recombinant native and variant human alpha 1-antichymotrypsins.

Authors: H Rubin; Z M Wang; E B Nickbarg; S McLarney; N Naidoo; O L Schoenberger; J L Johnson; B S Cooperman
Journal: J Biol Chem Date: 1990-01-15 Impact factor: 5.157

Alpha-1-antichymotrypsin variant detected by PCR-single strand conformation polymorphism (PCR-SSCP) and direct sequencing.

1. Detection of a new mutant alpha-1-antichymotrypsin in patients with occlusive-cerebrovascular disease.

2. Cloning, expression, purification, and biological activity of recombinant native and variant human alpha 1-antichymotrypsins.

3. Enhancement by alpha-1-antichymotrypsin of antibody response in vivo.

4. Effect of alpha-1-antichymotrypsin on activity of DNA primase isolated from human stomach adenocarcinoma cells.

5. Human alpha-1-antichymotrypsin: interaction with chymotrypsin-like proteinases.

6. Inhibition of human natural cytotoxicity by macromolecular antiproteases.

7. Sequence homology between human alpha 1-antichymotrypsin, alpha 1-antitrypsin, and antithrombin III.

8. Inhibition of human DNA polymerase alpha by alpha 1-antichymotrypsin.

9. Purification, properties and identification of a serum DNA binding protein (64DP) and its microheterogeneity.