Literature DB >> 1328684

cDNA clone of hepatitis A virus encoding a virulent virus: induction of viral hepatitis by direct nucleic acid transfection of marmosets.

S U Emerson1, M Lewis, S Govindarajan, M Shapiro, T Moskal, R H Purcell.   

Abstract

Direct inoculation of marmoset livers with an in vitro transcription mixture containing cDNA and full-length genomic RNA transcripts of hepatitis A virus resulted in acute viral hepatitis. Elevations in serum levels of liver enzymes were correlated with appearance of antibody to hepatitis A virus. Genomes of infectious hepatitis A virus isolated from the feces of transfected marmosets contained the same mutation as the cDNA template used for transfection. Liver biopsies confirmed that the virus encoded by the cDNA clone induced histopathological changes equivalent to those caused by virulent wild-type virus.

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Year:  1992        PMID： 1328684      PMCID： PMC240161     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  17 in total

1.  Antigenic and genetic variation in cytopathic hepatitis A virus variants arising during persistent infection: evidence for genetic recombination.

Authors:  S M Lemon; P C Murphy; P A Shields; L H Ping; S M Feinstone; T Cromeans; R W Jansen
Journal:  J Virol       Date:  1991-04       Impact factor: 5.103

2.  Mutations in both the 2B and 2C genes of hepatitis A virus are involved in adaptation to growth in cell culture.

Authors:  S U Emerson; Y K Huang; C McRill; M Lewis; R H Purcell
Journal:  J Virol       Date:  1992-02       Impact factor: 5.103

3.  Attenuation and cell culture adaptation of hepatitis A virus (HAV): a genetic analysis with HAV cDNA.

Authors:  J I Cohen; B Rosenblum; S M Feinstone; J Ticehurst; R H Purcell
Journal:  J Virol       Date:  1989-12       Impact factor: 5.103

4.  Nucleotide sequence of high-passage hepatitis A virus strain HM175: comparison with wild-type and cell culture-adapted strains.

Authors:  B C Ross; B N Anderson; P C Edwards; I D Gust
Journal:  J Gen Virol       Date:  1989-10       Impact factor: 3.891

5.  Identification and characterization of the virus causing rabbit hemorrhagic disease.

Authors:  V F Ohlinger; B Haas; G Meyers; F Weiland; H J Thiel
Journal:  J Virol       Date:  1990-07       Impact factor: 5.103

6.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

Authors:  P Chomczynski; N Sacchi
Journal:  Anal Biochem       Date:  1987-04       Impact factor: 3.365

7.  Genomic regions of neurovirulence and attenuation in Theiler murine encephalomyelitis virus.

Authors:  M A Calenoff; K S Faaberg; H L Lipton
Journal:  Proc Natl Acad Sci U S A       Date:  1990-02       Impact factor: 11.205

8.  Neurovirulence determinants of genetically engineered Theiler viruses.

Authors:  J L Fu; S Stein; L Rosenstein; T Bodwell; M Routbort; B L Semler; R P Roos
Journal:  Proc Natl Acad Sci U S A       Date:  1990-06       Impact factor: 11.205

9.  Identification of two determinants that attenuate vaccine-related type 2 poliovirus.

Authors:  R B Ren; E G Moss; V R Racaniello
Journal:  J Virol       Date:  1991-03       Impact factor: 5.103

10.  Propagation of human hepatitis A virus in African green monkey kidney cell culture: primary isolation and serial passage.

Authors:  R J Daemer; S M Feinstone; I D Gust; R H Purcell
Journal:  Infect Immun       Date:  1981-04       Impact factor: 3.441
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  19 in total

1.  Transfection of single-stranded hepatitis A virus RNA activates MHC class I pathway.

Authors:  K Suzuki; M Yanagi; A Mori-Aoki; E Moriyama; K J Ishii; L D Kohn
Journal:  Clin Exp Immunol       Date:  2002-02       Impact factor: 4.330

2.  Identification of VP1/2A and 2C as virulence genes of hepatitis A virus and demonstration of genetic instability of 2C.

Authors:  Suzanne U Emerson; Ying K Huang; Hanh Nguyen; Alicia Brockington; Sugantha Govindarajan; Marisa St Claire; Max Shapiro; Robert H Purcell
Journal:  J Virol       Date:  2002-09       Impact factor: 5.103

3.  Immunoglobulin A (IgA) is a natural ligand of hepatitis A virus cellular receptor 1 (HAVCR1), and the association of IgA with HAVCR1 enhances virus-receptor interactions.

Authors:  Cecilia Tami; Erica Silberstein; Mohanraj Manangeeswaran; Gordon J Freeman; Sarah E Umetsu; Rosemarie H DeKruyff; Dale T Umetsu; Gerardo G Kaplan
Journal:  J Virol       Date:  2007-01-17       Impact factor: 5.103

4.  Stable growth of wild-type hepatitis A virus in cell culture.

Authors:  Krishnamurthy Konduru; Gerardo G Kaplan
Journal:  J Virol       Date:  2006-02       Impact factor: 5.103

5.  Vectorial entry and release of hepatitis A virus in polarized human hepatocytes.

Authors:  Michelle J Snooks; Purnima Bhat; Jason Mackenzie; Natalie A Counihan; Nicola Vaughan; David A Anderson
Journal:  J Virol       Date:  2008-06-25       Impact factor: 5.103

6.  Recombinant hepatitis E virus genomes infectious for primates: importance of capping and discovery of a cis-reactive element.

Authors:  S U Emerson; M Zhang; X J Meng; H Nguyen; M St Claire; S Govindarajan; Y K Huang; R H Purcell
Journal:  Proc Natl Acad Sci U S A       Date:  2001-12-11       Impact factor: 11.205

7.  Progress toward the development of a genetically engineered attenuated hepatitis A virus vaccine.

Authors:  A W Funkhouser; G Raychaudhuri; R H Purcell; S Govindarajan; R Elkins; S U Emerson
Journal:  J Virol       Date:  1996-11       Impact factor: 5.103

8.  Hepatitis A viruses with deletions in the 2A gene are infectious in cultured cells and marmosets.

Authors:  S A Harmon; S U Emerson; Y K Huang; D F Summers; E Ehrenfeld
Journal:  J Virol       Date:  1995-09       Impact factor: 5.103

9.  Transcripts from a single full-length cDNA clone of hepatitis C virus are infectious when directly transfected into the liver of a chimpanzee.

Authors:  M Yanagi; R H Purcell; S U Emerson; J Bukh
Journal:  Proc Natl Acad Sci U S A       Date:  1997-08-05       Impact factor: 11.205

10.  A new Chinese isolate of hepatitis E virus: comparison with strains recovered from different geographical regions.

Authors:  S Yin; R H Purcell; S U Emerson
Journal:  Virus Genes       Date:  1994-09       Impact factor: 2.332
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