Literature DB >> 1324757

A triple-resonance pulse scheme for selectively correlating amide 1HN and 15N nuclei with the 1H alpha proton of the preceding residue.

R T Clubb1, G Wagner.   

Abstract

A 3D 1H-15N-13C triple resonance experiment is presented that contains exclusively cross peaks between the 1HN and 15N nuclei of one residue with the H alpha of the preceding residue. The pulse sequence, designed to minimize the time coherence, is transverse on nuclei with short T2 values. The experiment consists of coherence transfers via one-bond couplings from the HN via N, CO, C alpha to the H alpha and back to the HN for detection; it is called HN(COCA)HA. The experiment was tested on uniformly 15N- and 13C-enriched T4 lysozyme.

Entities:  

Mesh：

Substances：

Year:  1992        PMID： 1324757     DOI： 10.1007/bf01874816

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  6 in total

1.  An efficient 3D NMR technique for correlating the proton and 15N backbone amide resonances with the alpha-carbon of the preceding residue in uniformly 15N/13C enriched proteins.

Authors:  A Bax; M Ikura
Journal:  J Biomol NMR       Date:  1991-05       Impact factor: 2.835

2.  Sequential resonance assignments in protein 1H nuclear magnetic resonance spectra. Basic pancreatic trypsin inhibitor.

Authors:  G Wagner; K Wüthrich
Journal:  J Mol Biol       Date:  1982-03-05       Impact factor: 5.469

3.  Application of phase sensitive two-dimensional correlated spectroscopy (COSY) for measurements of 1H-1H spin-spin coupling constants in proteins.

Authors:  D Marion; K Wüthrich
Journal:  Biochem Biophys Res Commun       Date:  1983-06-29       Impact factor: 3.575

4.  Assignment of the backbone 1H and 15N NMR resonances of bacteriophage T4 lysozyme.

Authors:  L P McIntosh; A J Wand; D F Lowry; A G Redfield; F W Dahlquist
Journal:  Biochemistry       Date:  1990-07-10       Impact factor: 3.162

5.  A new 3D HN(CA)HA experiment for obtaining fingerprint HN-Halpha peaks in 15N- and 13C-labeled proteins.

Authors:  R T Clubb; V Thanabal; G Wagner
Journal:  J Biomol NMR       Date:  1992-03       Impact factor: 2.835

6.  A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin.

Authors:  M Ikura; L E Kay; A Bax
Journal:  Biochemistry       Date:  1990-05-15       Impact factor: 3.162
  6 in total
  8 in total

1.  Application of multiple-quantum line narrowing with simultaneous 1H and 13C constant-time scalar-coupling evolution in PFG-HACANH and PFG-HACA(CO)NH triple-resonance experiments.

Authors:  G V Swapna; C B Rios; Z Shang; G T Montelione
Journal:  J Biomol NMR       Date:  1997-01       Impact factor: 2.835

Review 2.  Resonance assignment strategies for the analysis of NMR spectra of proteins.

Authors:  M F Leopold; J L Urbauer; A J Wand
Journal:  Mol Biotechnol       Date:  1994-08       Impact factor: 2.695

3.  A tracked approach for automated NMR assignments in proteins (TATAPRO).

Authors:  H S Atreya; S C Sahu; K V Chary; G Govil
Journal:  J Biomol NMR       Date:  2000-06       Impact factor: 2.835

4.  Automated probabilistic method for assigning backbone resonances of (13C,15N)-labeled proteins.

Authors:  J A Lukin; A P Gove; S N Talukdar; C Ho
Journal:  J Biomol NMR       Date:  1997-02       Impact factor: 2.835

5.  Phase labeling of C-H and C-C spin-system topologies: application in constant-time PFG-CBCA(CO)NH experiments for discriminating amino acid spin-system types.

Authors:  C B Rios; W Feng; M Tashiro; Z Shang; G T Montelione
Journal:  J Biomol NMR       Date:  1996-10       Impact factor: 2.835

6.  3D triple-resonance NMR techniques for the sequential assignment of NH and 15N resonances in 15N- and 13C-labelled proteins.

Authors:  R Weisemann; H Rüterjans; W Bermel
Journal:  J Biomol NMR       Date:  1993-01       Impact factor: 2.835

7.  Disorder and structure in the Rab11 binding domain of Rab11 family interacting protein 2.

Authors:  Jie Wei; Yuqi Liu; Kakoli Bose; Gillian D Henry; James D Baleja
Journal:  Biochemistry       Date:  2009-01-27       Impact factor: 3.162

8.  High-resolution detection of five frequencies in a single 3D spectrum: HNHCACO--a bidirectional coherence transfer experiment.

Authors:  Y Pang; L Zeng; A V Kurochkin; E R Zuiderweg
Journal:  J Biomol NMR       Date:  1998-02       Impact factor: 2.835
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.