Literature DB >> 1324234

Direct detection of Epstein-Barr virus DNA from a single Reed-Sternberg cell of Hodgkin's disease by polymerase chain reaction.

N Teramoto1, T Akagi, T Yoshino, K Takahashi, H J Jeon.   

Abstract

Eleven cases of Hodgkin's disease (HD) were examined for the presence of the Epstein-Barr virus (EBV) genome, using the polymerase chain reaction (PCR) to detect EBV DNA in whole paraffin-embedded tissue specimens and in single cells picked out from the specimens with a micromanipulator. The EBV genome was detected in 5 of the 11 cases by conventional PCR. Single cell PCR demonstrated the EBV genome in Reed-Sternberg cells from all the EBV-positive cases, but not from any of the EBV-negative cases. Background lymphocytes and lysozyme-positive histiocytes from EBV-positive cases did not contain the EBV genome. These results indicate an etiological association of EBV with some cases of HD.

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Year:  1992        PMID： 1324234      PMCID： PMC5918838          DOI： 10.1111/j.1349-7006.1992.tb00110.x

Source DB:  PubMed          Journal:  Jpn J Cancer Res        ISSN： 0910-5050


  14 in total

1.  Epstein-Barr viral genome in lymph nodes from patients with Hodgkin's disease may not be specific to Reed-Sternberg cells.

Authors:  A Masih; D Weisenburger; M Duggan; J Armitage; R Bashir; D Mitchell; R Wickert; D T Purtilo
Journal:  Am J Pathol       Date:  1991-07       Impact factor: 4.307

2.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

3.  Demonstration of Epstein-Barr viral DNA in formalin-fixed, paraffin-embedded samples of Hodgkin's disease.

Authors:  C M Libetta; J H Pringle; C A Angel; A W Craft; A J Malcolm; I Lauder
Journal:  J Pathol       Date:  1990-07       Impact factor: 7.996

4.  Epstein-Barr viral DNA in tissues of Hodgkin's disease.

Authors:  L M Weiss; J G Strickler; R A Warnke; D T Purtilo; J Sklar
Journal:  Am J Pathol       Date:  1987-10       Impact factor: 4.307

5.  Amplification of human minisatellites by the polymerase chain reaction: towards DNA fingerprinting of single cells.

Authors:  A J Jeffreys; V Wilson; R Neumann; J Keyte
Journal:  Nucleic Acids Res       Date:  1988-12-09       Impact factor: 16.971

6.  A new and improved method for 3'-end labelling DNA using [alpha-32P]ddATP.

Authors:  S I Yousaf; A R Carroll; B E Clarke
Journal:  Gene       Date:  1984-03       Impact factor: 3.688

7.  Epstein-Barr virus status and tumour cell phenotype in sporadic Burkitt's lymphoma.

Authors:  M Rowe; C M Rooney; C F Edwards; G M Lenoir; A B Rickinson
Journal:  Int J Cancer       Date:  1986-03-15       Impact factor: 7.396

8.  Analysis of DNA in fresh and fixed tissue by the polymerase chain reaction.

Authors:  B B Rogers; L C Alpert; E A Hine; G J Buffone
Journal:  Am J Pathol       Date:  1990-03       Impact factor: 4.307

9.  High incidence of Epstein-Barr virus genomes in Hodgkin's disease.

Authors:  H Herbst; G Niedobitek; M Kneba; M Hummel; T Finn; I Anagnostopoulos; M Bergholz; G Krieger; H Stein
Journal:  Am J Pathol       Date:  1990-07       Impact factor: 4.307

10.  Demonstration of monoclonal EBV genomes in Hodgkin's disease and Ki-1-positive anaplastic large cell lymphoma by combined Southern blot and in situ hybridization.

Authors:  I Anagnostopoulos; H Herbst; G Niedobitek; H Stein
Journal:  Blood       Date:  1989-08-01       Impact factor: 22.113
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  4 in total

1.  Hodgkin's disease: immunoglobulin heavy and light chain gene rearrangements revealed in single Hodgkin/Reed-Sternberg cells.

Authors:  F Deng; G Lü; G Li; G Yang
Journal:  Mol Pathol       Date:  1999-02

2.  Microdissection and polymerase chain reaction amplification of genomic DNA from histological tissue sections.

Authors:  C A Moskaluk; S E Kern
Journal:  Am J Pathol       Date:  1997-05       Impact factor: 4.307

3.  Infectivity of human T-lymphotropic virus type I to human nervous tissue cells in vitro.

Authors:  T Akagi; Y Hoshida; T Yoshino; N Teramoto; E Kondo; K Hayashi; K Takahashi
Journal:  Acta Neuropathol       Date:  1992       Impact factor: 17.088

4.  Recent Advances in the Pathobiology of Hodgkin's Lymphoma: Potential Impact on Diagnostic, Predictive, and Therapeutic Strategies.

Authors:  Diponkar Banerjee
Journal:  Adv Hematol       Date:  2011-01-18
  4 in total

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