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Literature DB >> 1311075

The recognition of DNA cleavage sites by porcine spleen topoisomerase II.

Abstract

The cutting sites specificity of topoisomerase II from porcine spleen were determined by a modified Sanger's DNA sequencing method. The topoisomerase II prefers to cut DNA at the 3' side of A and leave 5' protruding end with two staggering bases. Through the free energy analysis for DNA duplex, we also found that the topoisomerase II seemed cut DNA preferably at energetically unstable regions. So it is concluded that the specific DNA cutting by porcine spleen topoisomerase II has two structural recognition factors: one is to localize around the energetically unstable region and another is to act at the 3' side of A base.

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Year: 1992 PMID： 1311075 PMCID： PMC310409 DOI： 10.1093/nar/20.3.467

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

33 in total

1. Identification within the simian virus 40 genome of a chromosomal loop attachment site that contains topoisomerase II cleavage sites.

Authors: Y Pommier; P N Cockerill; K W Kohn; W T Garrard
Journal: J Virol Date: 1990-01 Impact factor: 5.103

2. Dysfunction of chromosomal loop attachment sites: illegitimate recombination linked to matrix association regions and topoisomerase II.

Authors: A O Sperry; V C Blasquez; W T Garrard
Journal: Proc Natl Acad Sci U S A Date: 1989-07 Impact factor: 11.205

The recognition of DNA cleavage sites by porcine spleen topoisomerase II.

1. Identification within the simian virus 40 genome of a chromosomal loop attachment site that contains topoisomerase II cleavage sites.

2. Dysfunction of chromosomal loop attachment sites: illegitimate recombination linked to matrix association regions and topoisomerase II.

3. A new compound, withangulatin A, promotes type II DNA topoisomerase-mediated DNA damage.

4. A rapid single-stranded cloning, sequencing, insertion, and deletion strategy.

5. DNA topoisomerase II is required at the time of mitosis in yeast.

6. Sequencing of RNA transcripts synthesized in vitro from plasmids containing bacteriophage promoters.

7. Stability of ribonucleic acid double-stranded helices.

8. Illegitimate recombination mediated by calf thymus DNA topoisomerase II in vitro.

9. Local sequence requirements for DNA cleavage by mammalian topoisomerase II in the presence of doxorubicin.

10. Single strand DNA cleavage reaction of duplex DNA by Drosophila topoisomerase II.

Review 1. Multilevel therapeutic targeting by topoisomerase inhibitors.