Literature DB >> 1309999

Expression of feline immunodeficiency virus gag gene in Escherichia coli.

T Furuya1, A Hasegawa, M Saitoh, T Miyazawa, Y Tohya, M Hayami, E Takahashi, K Miki, T Mikami.   

Abstract

The gag gene of a Japanese feline immunodeficiency virus (FIV) isolate, designated as FIV TM 2, was expressed in Escherichia coli as a fusion protein with TrpE. Using this expressed protein, an enzyme-linked immunosorbent assay was developed for detection of antibodies to FIV gag protein in feline sera. With serum samples from a cat experimentally infected with FIV, it was demonstrated that the period of seroconversion detected by this method corresponded to that by Western blotting.

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Year:  1992        PMID： 1309999     DOI： 10.1007/bf01317200

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  20 in total

1.  Continuous production of feline immunodeficiency virus in a feline T-lymphoblastoid cell line (MYA-1 cells).

Authors:  T Miyazawa; Y Kawaguchi; T Furuya; S Itagaki; E Takahashi; T Mikami
Journal:  Nihon Juigaku Zasshi       Date:  1990-08

2.  The cloning and expression in Escherichia coli of sequences coding for p24, the core protein of human immunodeficiency virus, and the use of the recombinant protein in characterizing a panel of monoclonal antibodies against the viral p24 protein.

Authors:  R P Spence; W M Jarvill; R B Ferns; R S Tedder; D Parker
Journal:  J Gen Virol       Date:  1989-11       Impact factor: 3.891

3.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

4.  Oligonucleotide-directed construction of mutations via gapped duplex DNA.

Authors:  W Kramer; H J Fritz
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

5.  The gapped duplex DNA approach to oligonucleotide-directed mutation construction.

Authors:  W Kramer; V Drutsa; H W Jansen; B Kramer; M Pflugfelder; H J Fritz
Journal:  Nucleic Acids Res       Date:  1984-12-21       Impact factor: 16.971

6.  The expression in Escherichia coli of sequences coding for the p18 protein of human immunodeficiency virus and the use of the recombinant protein in characterizing a panel of monoclonal antibodies against the viral p18 protein.

Authors:  R P Spence; J Walker; W M Jarvill; R B Ferns; R S Tedder; Q Sattentau; J Weber; N R Parry; P E Highfield
Journal:  J Gen Virol       Date:  1989-11       Impact factor: 3.891

7.  Human T-cell lymphotropic virus type III (HTLV-III) core antigens: synthesis in Escherichia coli and immunoreactivity with human sera.

Authors:  J Ghrayeb; I Kato; S McKinney; J J Huang; P K Chanda; D D Ho; M G Sarangadharan; T W Chang; N T Chang
Journal:  DNA       Date:  1986-04

8.  Serological responses of cats to feline immunodeficiency virus.

Authors:  M J Hosie; O Jarrett
Journal:  AIDS       Date:  1990-03       Impact factor: 4.177

9.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

10.  Molecular characterization and heterogeneity of feline immunodeficiency virus isolates.

Authors:  N Maki; T Miyazawa; M Fukasawa; A Hasegawa; M Hayami; K Miki; T Mikami
Journal:  Arch Virol       Date:  1992       Impact factor: 2.574
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  3 in total

1.  Roles of the auxiliary genes and AP-1 binding site in the long terminal repeat of feline immunodeficiency virus in the early stage of infection in cats.

Authors:  Y Inoshima; M Kohmoto; Y Ikeda; H Yamada; Y Kawaguchi; K Tomonaga; T Miyazawa; C Kai; T Umemura; T Mikami
Journal:  J Virol       Date:  1996-12       Impact factor: 5.103

2.  Replicative difference in early-passage feline brain cells among feline immunodeficiency virus isolates.

Authors:  Y Kawaguchi; K Maeda; Y Tohya; T Furuya; T Miyazawa; T Horimoto; J Norimine; C Kai; T Mikami
Journal:  Arch Virol       Date:  1992       Impact factor: 2.574

3.  Detection of anti-gag antibodies of feline immunodeficiency virus in cat sera by enzyme-linked immunosorbent assay.

Authors:  T Furuya; A Hasegawa; T Miyazawa; K Miki; T Mikami
Journal:  Arch Virol       Date:  1992       Impact factor: 2.574
  3 in total

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