Literature DB >> 1318710

Detection of anti-gag antibodies of feline immunodeficiency virus in cat sera by enzyme-linked immunosorbent assay.

T Furuya1, A Hasegawa, T Miyazawa, K Miki, T Mikami.   

Abstract

Using gag protein of feline immunodeficiency virus (FIV) expressed in Escherichia coli, an enzyme-linked immunosorbent assay (ELISA) system was developed for detection of antibodies to FIV gag protein in cat sera. With serum samples from cats experimentally infected with several strains and an infectious molecular clone of FIV, increases of the antibody titers to FIV gag protein were observed in all cases by the ELISA at early stage of infection. When we examined a total of 415 field cat sera which were previously tested by an indirect immunofluorescence assay (IFA), 9 (12.9%) out of 70 IFA positive sera were judged as negative by the ELISA. However, all 3 serum samples tested among the 9 IFA positive sera had antibodies to gp130 but not to p26 by a radioimmunoprecipitation assay. The results indicated that some IFA positive sera did not have antibodies to the p26 though they have antibodies to other proteins specific for FIV.

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Year:  1992        PMID： 1318710     DOI： 10.1007/bf01309815

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  23 in total

1.  Nucleotide sequence analysis of feline immunodeficiency virus: genome organization and relationship to other lentiviruses.

Authors:  R A Olmsted; V M Hirsch; R H Purcell; P R Johnson
Journal:  Proc Natl Acad Sci U S A       Date:  1989-10       Impact factor: 11.205

2.  Comparison of two host cell range variants of feline immunodeficiency virus.

Authors:  T R Phillips; R L Talbott; C Lamont; S Muir; K Lovelace; J H Elder
Journal:  J Virol       Date:  1990-10       Impact factor: 5.103

3.  Existence of feline immunodeficiency virus infection in Japanese cat population since 1968.

Authors:  T Furuya; Y Kawaguchi; T Miyazawa; Y Fujikawa; Y Tohya; M Azetaka; E Takahashi; T Mikami
Journal:  Nihon Juigaku Zasshi       Date:  1990-08

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Isolation of a T-lymphotropic lentivirus from a persistently leucopenic domestic cat.

Authors:  D A Harbour; P D Williams; T J Gruffydd-Jones; J Burbridge; G R Pearson
Journal:  Vet Rec       Date:  1988-01-23       Impact factor: 2.695

6.  Serological evidence of feline immunodeficiency virus infection in UK cats from 1975-76.

Authors:  T J Gruffydd-Jones; C D Hopper; D A Harbour; H Lutz
Journal:  Vet Rec       Date:  1988-11-26       Impact factor: 2.695

7.  Molecular cloning of feline immunodeficiency virus.

Authors:  R A Olmsted; A K Barnes; J K Yamamoto; V M Hirsch; R H Purcell; P R Johnson
Journal:  Proc Natl Acad Sci U S A       Date:  1989-04       Impact factor: 11.205

8.  Pathogenesis of experimentally induced feline immunodeficiency virus infection in cats.

Authors:  J K Yamamoto; E Sparger; E W Ho; P R Andersen; T P O'Connor; C P Mandell; L Lowenstine; R Munn; N C Pedersen
Journal:  Am J Vet Res       Date:  1988-08       Impact factor: 1.156

9.  Molecular characterization and heterogeneity of feline immunodeficiency virus isolates.

Authors:  N Maki; T Miyazawa; M Fukasawa; A Hasegawa; M Hayami; K Miki; T Mikami
Journal:  Arch Virol       Date:  1992       Impact factor: 2.574

10.  Preliminary comparisons of the biological properties of two strains of feline immunodeficiency virus (FIV) isolated in Japan with FIV Petaluma strain isolated in the United States.

Authors:  T Miyazawa; T Furuya; S Itagaki; Y Tohya; K Nakano; E Takahashi; T Mikami
Journal:  Arch Virol       Date:  1989       Impact factor: 2.574
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  5 in total

1.  Rapid immunochromatographic test using recombinant SAG2 for detection of antibodies against Toxoplasma gondii in cats.

Authors:  Xiaohong Huang; Xuenan Xuan; Haruyuki Hirata; Naoaki Yokoyama; Longshan Xu; Naoyoshi Suzuki; Ikuo Igarashi
Journal:  J Clin Microbiol       Date:  2004-01       Impact factor: 5.948

2.  Characterization of Toxoplasma gondii SAG2 expressed in insect cells by recombinant baculovirus and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay.

Authors:  Xiaohong Huang; Xuenan Xuan; Hiroshi Suzuki; Chihiro Sugimoto; Hideyuki Nagasawa; Kozo Fujisaki; Takeshi Mikami; Ikuo Igarashi
Journal:  Clin Diagn Lab Immunol       Date:  2002-11

3.  Expression of feline immunodeficiency virus gag and env precursor proteins in Spodoptera frugiperda cells and their use in immunodiagnosis.

Authors:  E J Verschoor; A L van Vliet; H F Egberink; W Hesselink; M C Horzinek; A de Ronde
Journal:  J Clin Microbiol       Date:  1993-09       Impact factor: 5.948

Review 4.  Feline immunodeficiency virus: an interesting model for AIDS studies and an important cat pathogen.

Authors:  M Bendinelli; M Pistello; S Lombardi; A Poli; C Garzelli; D Matteucci; L Ceccherini-Nelli; G Malvaldi; F Tozzini
Journal:  Clin Microbiol Rev       Date:  1995-01       Impact factor: 26.132

5.  Localization of the viral antigen of feline immunodeficiency virus in the lymph nodes of cats at the early stage of infection.

Authors:  T Toyosaki; T Miyazawa; T Furuya; K Tomonaga; Y S Shin; M Okita; Y Kawaguchi; C Kai; S Mori; T Mikami
Journal:  Arch Virol       Date:  1993       Impact factor: 2.574
  5 in total

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