Literature DB >> 8187795

Detection of JC virus by polymerase chain reaction and colorimetric DNA hybridization assay.

C Vignoli1, X De Lamballerie, C Zandotti, C Tamalet, P De Micco.   

Abstract

A procedure for rapid detection of JC virus (JCV) using the polymerase chain reaction is described. The procedure was tested in eight HIV-1-seropositive patients with progressive multifocal leukoencephalopathy. One-step DNA extraction using a chelating resin was carried out on clinical samples of cerebrospinal fluid (CSF), urine and brain tissue. After amplification, PCR products were detected by a DNA hybridization method. Microplates were coated with a specific probe and hybridized PCR products were revealed by a commercial colorimetric immunoassay. Using this procedure JC virus DNA was detected in all CSF specimens from patients with progressive multifocal leukoencephalopathy. This sensitive and rapid (24 h) procedure could greatly facilitate use of the DNA probe assay for detection of JC virus in clinical laboratories.

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Year:  1993        PMID： 8187795     DOI： 10.1007/bf01992174

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN： 0934-9723            Impact factor:   3.267


  13 in total

1.  Progressive multifocal leukoencephalopathy.

Authors:  E P RICHARDSON
Journal:  N Engl J Med       Date:  1961-10-26       Impact factor: 91.245

2.  Amplification of JC virus DNA from brain and cerebrospinal fluid of patients with progressive multifocal leukoencephalopathy.

Authors:  J Henson; M Rosenblum; D Armstrong; H Furneaux
Journal:  Neurology       Date:  1991-12       Impact factor: 9.910

3.  Survey of urine from transplant recipients for polyomaviruses JC and BK using the polymerase chain reaction.

Authors:  W F Marshall; A Telenti; J Proper; A J Aksamit; T F Smith
Journal:  Mol Cell Probes       Date:  1991-04       Impact factor: 2.365

4.  A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification.

Authors:  X de Lamballerie; C Zandotti; C Vignoli; C Bollet; P de Micco
Journal:  Res Microbiol       Date:  1992-10       Impact factor: 3.992

5.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

Authors:  R K Saiki; S Scharf; F Faloona; K B Mullis; G T Horn; H A Erlich; N Arnheim
Journal:  Science       Date:  1985-12-20       Impact factor: 47.728

6.  Progressive multifocal leukoencephalopathy: investigation of three cases using in situ hybridization with JC virus biotinylated DNA probe.

Authors:  A J Aksamit; P Mourrain; J L Sever; E O Major
Journal:  Ann Neurol       Date:  1985-10       Impact factor: 10.422

7.  Cultivation of papova-like virus from human brain with progressive multifocal leucoencephalopathy.

Authors:  B L Padgett; D L Walker; G M ZuRhein; R J Eckroade; B H Dessel
Journal:  Lancet       Date:  1971-06-19       Impact factor: 79.321

8.  DNA enzyme immunoassay: general method for detecting products of polymerase chain reaction.

Authors:  G Mantero; A Zonaro; A Albertini; P Bertolo; D Primi
Journal:  Clin Chem       Date:  1991-03       Impact factor: 8.327

9.  Detection of BK virus and JC virus in urine and brain tissue by the polymerase chain reaction.

Authors:  R R Arthur; S Dagostin; K V Shah
Journal:  J Clin Microbiol       Date:  1989-06       Impact factor: 5.948

Review 10.  Progressive multifocal leukoencephalopathy associated with human immunodeficiency virus infection. A review of the literature with a report of sixteen cases.

Authors:  J R Berger; B Kaszovitz; M J Post; G Dickinson
Journal:  Ann Intern Med       Date:  1987-07       Impact factor: 25.391
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  2 in total

1.  Latency and reactivation of JC virus in peripheral blood of human immunodeficiency virus type 1-infected patients.

Authors:  V Dubois; H Dutronc; M E Lafon; V Poinsot; J L Pellegrin; J M Ragnaud; A M Ferrer; H J Fleury
Journal:  J Clin Microbiol       Date:  1997-09       Impact factor: 5.948

2.  Evaluation of performances of three DNA enzyme immunoassays for detection of Helicobacter pylori PCR products from biopsy specimens.

Authors:  L Monteiro; J Cabrita; F Mégraud
Journal:  J Clin Microbiol       Date:  1997-11       Impact factor: 5.948
  2 in total

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