Literature DB >> 12958287

Development of a 5' nuclease-based real-time PCR assay for quantitative detection of cariogenic dental pathogens Streptococcus mutans and Streptococcus sobrinus.

Akihiro Yoshida1, Nao Suzuki, Yoshio Nakano, Miki Kawada, Takahiko Oho, Toshihiko Koga.   

Abstract

A 5' nuclease TaqMan PCR assay was developed for the quantitative detection of the major cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus. The absolute and relative numbers of bacteria were measured by this method. This assay will be useful for quantifying these organisms in oral specimens and for analyzing biofilm formation.

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Year:  2003        PMID： 12958287      PMCID： PMC193796          DOI： 10.1128/JCM.41.9.4438-4441.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  21 in total

1.  Identification of Actinobacillus actinomycetemcomitans serotypes by multiplex PCR.

Authors:  N Suzuki; Y Nakano; Y Yoshida; D Ikeda; T Koga
Journal:  J Clin Microbiol       Date:  2001-05       Impact factor: 5.948

Review 2.  Identifying in vivo expressed streptococcal genes in endocarditis.

Authors:  L Tao; M C Herzberg
Journal:  Methods Enzymol       Date:  1999       Impact factor: 1.600

3.  Development of a 5' fluorogenic nuclease-based real-time PCR assay for quantitative detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis.

Authors:  Akihiro Yoshida; Nao Suzuki; Yoshio Nakano; Takahiko Oho; Miki Kawada; Toshihiko Koga
Journal:  J Clin Microbiol       Date:  2003-02       Impact factor: 5.948

4.  Microbial variations in approximal dental plaque.

Authors:  G H Bowden; J M Hardie; G L Slack
Journal:  Caries Res       Date:  1975       Impact factor: 4.056

5.  Simple and rapid detection of Streptococcus mutans and Streptococcus sobrinus in human saliva by polymerase chain reaction.

Authors:  T Oho; Y Yamashita; Y Shimazaki; M Kushiyama; T Koga
Journal:  Oral Microbiol Immunol       Date:  2000-08

6.  PCR detection of Streptococcus mutans and S. sobrinus in dental plaque samples from Japanese pre-school children.

Authors:  Mitsugi Okada; Yoshiko Soda; Fumiko Hayashi; Takako Doi; Junji Suzuki; Kazuo Miura; Katsuyuki Kozai
Journal:  J Med Microbiol       Date:  2002-05       Impact factor: 2.472

Review 7.  Immunization against dental caries.

Authors:  Toshihiko Koga; Takahiko Oho; Yoshihiro Shimazaki; Yoshio Nakano
Journal:  Vaccine       Date:  2002-05-15       Impact factor: 3.641

Review 8.  Host-pathogen interactions in bacterial endocarditis: streptococcal virulence in the host.

Authors:  M C Herzberg; M W Meyer; A Kiliç; L Tao
Journal:  Adv Dent Res       Date:  1997-04

9.  Real-time PCR for quantification of Streptococcus mutans.

Authors:  Akira Yano; Noboru Kaneko; Hirohisa Ida; Toshikazu Yamaguchi; Nobuhiro Hanada
Journal:  FEMS Microbiol Lett       Date:  2002-11-19       Impact factor: 2.742

Review 10.  Virulence properties of oral bacteria: impact of molecular biology.

Authors:  H K Kuramitsu
Journal:  Curr Issues Mol Biol       Date:  2001-04       Impact factor: 2.081
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  55 in total

1.  Development of a real-time TaqMan assay to detect mendocina sublineage Pseudomonas species in contaminated metalworking fluids.

Authors:  Ratul Saha; Robert S Donofrio; Susan T Bagley
Journal:  J Ind Microbiol Biotechnol       Date:  2010-05-11       Impact factor: 3.346

2.  Real-time TaqMan PCR for quantifying oral bacteria during biofilm formation.

Authors:  Nao Suzuki; Yoshio Nakano; Akihiro Yoshida; Yoshihisa Yamashita; Yusuke Kiyoura
Journal:  J Clin Microbiol       Date:  2004-08       Impact factor: 5.948

3.  Genetic Diversity and Evidence for Transmission of Streptococcus mutans by DiversiLab rep-PCR.

Authors:  Stephanie S Momeni; Jennifer Whiddon; Kyounga Cheon; Tariq Ghazal; Stephen A Moser; Noel K Childers
Journal:  J Microbiol Methods       Date:  2016-07-16       Impact factor: 2.363

4.  Culture conditions of Roseobacter strain 27-4 affect its attachment and biofilm formation as quantified by real-time PCR.

Authors:  Jesper Bartholin Bruhn; Janus Anders Juul Haagensen; Dorthe Bagge-Ravn; Lone Gram
Journal:  Appl Environ Microbiol       Date:  2006-04       Impact factor: 4.792

5.  Repetitive extragenic palindromic PCR for study of Streptococcus mutans diversity and transmission in human populations.

Authors:  S A Moser; S C Mitchell; J D Ruby; S Momeni; R C Osgood; J Whiddon; N K Childers
Journal:  J Clin Microbiol       Date:  2009-12-09       Impact factor: 5.948

6.  Characteristics of Streptococcus mutans genotypes and dental caries in children.

Authors:  Kyounga Cheon; Stephen A Moser; Howard W Wiener; Jennifer Whiddon; Stephanie S Momeni; John D Ruby; Gary R Cutter; Noel K Childers
Journal:  Eur J Oral Sci       Date:  2013-04-19       Impact factor: 2.612

7.  Exploration of bacterial species associated with the salivary microbiome of individuals with a low susceptibility to dental caries.

Authors:  Haruna Yasunaga; Toru Takeshita; Yukie Shibata; Michiko Furuta; Yoshihiro Shimazaki; Sumio Akifusa; Toshiharu Ninomiya; Yutaka Kiyohara; Ichiro Takahashi; Yoshihisa Yamashita
Journal:  Clin Oral Investig       Date:  2016-12-24       Impact factor: 3.573

8.  Clinical and microbiological effects of ozone nano-bubble water irrigation as an adjunct to mechanical subgingival debridement in periodontitis patients in a randomized controlled trial.

Authors:  Sae Hayakumo; Shinichi Arakawa; Yoshihiro Mano; Yuichi Izumi
Journal:  Clin Oral Investig       Date:  2012-03-17       Impact factor: 3.573

9.  Oral microbiota in Swiss adolescents.

Authors:  Sigrun Eick; Malgorzata Pietkiewicz; Anton Sculean
Journal:  Clin Oral Investig       Date:  2012-02-28       Impact factor: 3.573

10.  A feasible enzyme-linked immunosorbent assay system using monoclonal and polyclonal antibodies against glucosyltransferase-B from Streptococcus mutans.

Authors:  Noriko Shinozaki-Kuwahara; Tomomi Hashizume-Takizawa; Masatomo Hirasawa; Kazuko Takada
Journal:  Hybridoma (Larchmt)       Date:  2012-06
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