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Literature DB >> 12574302

Development of a 5' fluorogenic nuclease-based real-time PCR assay for quantitative detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis.

Akihiro Yoshida¹, Nao Suzuki, Yoshio Nakano, Takahiko Oho, Miki Kawada, Toshihiko Koga.

Abstract

A 5' nuclease TaqMan PCR was developed for the quantitative detection of the periodontopathic bacteria Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. The relative numbers of bacteria were measured by the comparative threshold cycle method. This simplified method is a way of obtaining the relative quantities of these organisms from specimens and of monitoring the effect of therapy.

Entities: Disease Species

Mesh：

Substances：

Year: 2003 PMID： 12574302 PMCID： PMC149717 DOI： 10.1128/JCM.41.2.863-866.2003

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

19 in total

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Journal: J Clin Microbiol Date: 2001-05 Impact factor: 5.948

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Review 4. Virulence factors of Actinobacillus actinomycetemcomitans relevant to the pathogenesis of inflammatory periodontal diseases.

Authors: M Wilson; B Henderson
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Journal: Oral Microbiol Immunol Date: 2000-08

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8. Formation of methyl mercaptan from L-methionine by Porphyromonas gingivalis.

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9. Black-pigmented Bacteroides spp. in the human oral cavity.

Authors: J J Zambon; H S Reynolds; J Slots
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Review 10. Actinobacillus actinomycetemcomitans in human periodontal disease.

Authors: J J Zambon
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6. Subgingival Plaque in Periodontal Health Antagonizes at Toll-Like Receptor 4 and Inhibits E-Selectin Expression on Endothelial Cells.

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7. Toll-like receptor-2 and -4 responses regulate neutrophil infiltration into the junctional epithelium and significantly contribute to the composition of the oral microbiota.

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8. Use of the genomic subtractive hybridization technique to develop a real-time PCR assay for quantitative detection of Prevotella spp. in oral biofilm samples.

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10. Quantitative microbiological study of subgingival plaque by real-time PCR shows correlation between levels of Tannerella forsythensis and Fusobacterium spp.

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