Literature DB >> 12930953

Promoter choice affects the potency of HIV-1 specific RNA interference.

Daniel Boden1, Oliver Pusch, Fredrick Lee, Lynne Tucker, Peter R Shank, Bharat Ramratnam.   

Abstract

RNA interference (RNAi) is mediated by small interfering (si) RNAs that target and degrade mRNA in a sequence-specific manner. Cellular expression of siRNA can be achieved by the use of expression cassettes driven by RNA polymerase III (pol III) promoters. Here, we demonstrate that a modified tRNA(met)-derived (MTD) promoter effectively drives the cellular expression of HIV-1-specific siRNA. We observed up to 56% greater inhibition of virus production when the MTD promoter was used to drive the expression of short hairpin (sh) RNA targeting the HIV-1 transactivator protein tat compared to cassettes containing other pol III promoters such as H1, U6+1 and U6+27. We conclude that the MTD promoter is ideally suited to drive intracellular expression of HIV-1 specific siRNA and may serve as an important component of future RNAi vector delivery systems.

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Year:  2003        PMID： 12930953      PMCID： PMC212804          DOI： 10.1093/nar/gkg704

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  26 in total

1.  Secondary structure prediction and in vitro accessibility of mRNA as tools in the selection of target sites for ribozymes.

Authors:  M Amarzguioui; G Brede; E Babaie; M Grotli; B Sproat; H Prydz
Journal:  Nucleic Acids Res       Date:  2000-11-01       Impact factor: 16.971

2.  Specific interference with gene expression induced by long, double-stranded RNA in mouse embryonal teratocarcinoma cell lines.

Authors:  E Billy; V Brondani; H Zhang; U Müller; W Filipowicz
Journal:  Proc Natl Acad Sci U S A       Date:  2001-11-27       Impact factor: 11.205

3.  Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells.

Authors:  Patrick J Paddison; Amy A Caudy; Emily Bernstein; Gregory J Hannon; Douglas S Conklin
Journal:  Genes Dev       Date:  2002-04-15       Impact factor: 11.361

4.  The trans-activator gene of the human T cell lymphotropic virus type III is required for replication.

Authors:  A I Dayton; J G Sodroski; C A Rosen; W C Goh; W A Haseltine
Journal:  Cell       Date:  1986-03-28       Impact factor: 41.582

5.  RNA accessibility prediction: a theoretical approach is consistent with experimental studies in cell extracts.

Authors:  M Scherr; J J Rossi; G Sczakiel; V Patzel
Journal:  Nucleic Acids Res       Date:  2000-07-01       Impact factor: 16.971

6.  An unusually compact external promoter for RNA polymerase III transcription of the human H1RNA gene.

Authors:  E Myslinski; J C Amé; A Krol; P Carbon
Journal:  Nucleic Acids Res       Date:  2001-06-15       Impact factor: 16.971

7.  A system for stable expression of short interfering RNAs in mammalian cells.

Authors:  Thijn R Brummelkamp; René Bernards; Reuven Agami
Journal:  Science       Date:  2002-03-21       Impact factor: 47.728

8.  Effective expression of small interfering RNA in human cells.

Authors:  Cynthia P Paul; Paul D Good; Ira Winer; David R Engelke
Journal:  Nat Biotechnol       Date:  2002-05       Impact factor: 54.908

9.  Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells.

Authors:  Nan Sook Lee; Taikoh Dohjima; Gerhard Bauer; Haitang Li; Ming-Jie Li; Ali Ehsani; Paul Salvaterra; John Rossi
Journal:  Nat Biotechnol       Date:  2002-05       Impact factor: 54.908

10.  Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells.

Authors:  S M Elbashir; J Harborth; W Lendeckel; A Yalcin; K Weber; T Tuschl
Journal:  Nature       Date:  2001-05-24       Impact factor: 49.962
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  30 in total

1.  Nucleotide sequence homology requirements of HIV-1-specific short hairpin RNA.

Authors:  Oliver Pusch; Daniel Boden; Rebecca Silbermann; Fred Lee; Lynne Tucker; Bharat Ramratnam
Journal:  Nucleic Acids Res       Date:  2003-11-15       Impact factor: 16.971

Review 2.  Silence of the genes.

Authors:  Oded Singer; Amiel Yanai; Inder M Verma
Journal:  Proc Natl Acad Sci U S A       Date:  2004-04-05       Impact factor: 11.205

3.  Enhanced gene silencing of HIV-1 specific siRNA using microRNA designed hairpins.

Authors:  Daniel Boden; Oliver Pusch; Rebecca Silbermann; Fred Lee; Lynne Tucker; Bharat Ramratnam
Journal:  Nucleic Acids Res       Date:  2004-02-13       Impact factor: 16.971

Review 4.  Induction of RNA interference in dendritic cells.

Authors:  Mu Li; Hua Qian; Thomas E Ichim; Wei-Wen Ge; Igor A Popov; Katarzyna Rycerz; John Neu; David White; Robert Zhong; Wei-Ping Min
Journal:  Immunol Res       Date:  2004       Impact factor: 2.829

5.  Human U6 promoter drives stronger shRNA activity than its schistosome orthologue in Schistosoma mansoni and human fibrosarcoma cells.

Authors:  Raphaël Duvoisin; Mary A Ayuk; Gabriel Rinaldi; Sutas Suttiprapa; Victoria H Mann; Clarence M Lee; Nicola Harris; Paul J Brindley
Journal:  Transgenic Res       Date:  2011-09-28       Impact factor: 2.788

Review 6.  Applications of lentiviral vectors for shRNA delivery and transgenesis.

Authors:  Oded Singer; Inder M Verma
Journal:  Curr Gene Ther       Date:  2008-12       Impact factor: 4.391

7.  A significant increase of RNAi efficiency in human cells by the CMV enhancer with a tRNAlys promoter.

Authors:  Ma Weiwei; Xie Zhenhua; Liu Feng; Ning Hang; Jiang Yuyang
Journal:  J Biomed Biotechnol       Date:  2009-10-25

8.  Long-term transgene expression and inhibition of HIV-1 replication by a Cre/loxP-EBNA-1/oriP HIV-1-dependent ribozyme vector: Applications for HIV-1 gene therapy.

Authors:  Takashi Nagawa; Yuichiro Habu; Norihiko Matsumoto; Naoko Miyano-Kurosaki; Hiroshi Takaku
Journal:  J RNAi Gene Silencing       Date:  2006-01-13

9.  Vector-based siRNA delivery strategies for high-throughput screening of novel target genes.

Authors:  Meihong Chen; Quan Du; Hong-Yan Zhang; Claes Wahlestedt; Zicai Liang
Journal:  J RNAi Gene Silencing       Date:  2005-07-27

10.  A novel approach for inhibition of HIV-1 by RNA interference: counteracting viral escape with a second generation of siRNAs.

Authors:  Olivier ter Brake; Ben Berkhout
Journal:  J RNAi Gene Silencing       Date:  2005-10-14
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