Literature DB >> 11980967

Rapid PCR-based identification of methicillin-resistant Staphylococcus aureus from screening swabs.

D Jonas1, M Speck, F D Daschner, H Grundmann.   

Abstract

A PCR identification of methicillin-resistant Staphylococcus aureus (MRSA), obviating the need for subculture on agar media, was investigated. The combination of MRSA detection by mecA femB PCR with prior enrichment in selective broth was tested for 439 swabs. PCR identified 36 MRSA-positive samples, in concordance with conventional methods.

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Year:  2002        PMID： 11980967      PMCID： PMC130653          DOI： 10.1128/JCM.40.5.1821-1823.2002

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  17 in total

1.  Evaluation of the mecA femB duplex polymerase chain reaction for detection of methicillin-resistant Staphylococcus aureus.

Authors:  D Jonas; H Grundmann; D Hartung; F D Daschner; K J Towner
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1999-09       Impact factor: 3.267

2.  The competitive exclusion principle.

Authors:  G HARDIN
Journal:  Science       Date:  1960-04-29       Impact factor: 47.728

3.  Identification of methicillin-resistant staphylococci by multiplex polymerase chain reaction assay.

Authors:  S M Salisbury; L M Sabatini; C A Spiegel
Journal:  Am J Clin Pathol       Date:  1997-03       Impact factor: 2.493

4.  Soft salt-mannitol agar-cloxacillin test: a highly specific bedside screening test for detection of colonization with methicillin-resistant Staphylococcus aureus.

Authors:  N Mir; M Sánchez; F Baquero; B López; C Calderón; R Cantón
Journal:  J Clin Microbiol       Date:  1998-04       Impact factor: 5.948

5.  Comparison of ATB staph, rapid ATB staph, Vitek, and E-test methods for detection of oxacillin heteroresistance in staphylococci possessing mecA.

Authors:  N B Frebourg; D Nouet; L Lemée; E Martin; J F Lemeland
Journal:  J Clin Microbiol       Date:  1998-01       Impact factor: 5.948

6.  A major outbreak of methicillin-resistant Staphylococcus aureus caused by a new phage-type (EMRSA-16)

Authors:  R A Cox; C Conquest; C Mallaghan; R R Marples
Journal:  J Hosp Infect       Date:  1995-02       Impact factor: 3.926

7.  Detection of the mec-A gene and phenotypic detection of resistance in Staphylococcus aureus isolates with borderline or low-level methicillinresistance.

Authors:  G E Bignardi; N Woodford; A Chapman; A P Johnson; D C Speller
Journal:  J Antimicrob Chemother       Date:  1996-01       Impact factor: 5.790

8.  Multiplex PCR for identification of methicillin-resistant staphylococci in the clinical laboratory.

Authors:  D J Geha; J R Uhl; C A Gustaferro; D H Persing
Journal:  J Clin Microbiol       Date:  1994-07       Impact factor: 5.948

9.  Detection of mecA, femA, and femB genes in clinical strains of staphylococci using polymerase chain reaction.

Authors:  N Kobayashi; H Wu; K Kojima; K Taniguchi; S Urasawa; N Uehara; Y Omizu; Y Kishi; A Yagihashi; I Kurokawa
Journal:  Epidemiol Infect       Date:  1994-10       Impact factor: 2.451

10.  Rapid detection of methicillin-resistant Staphylococcus aureus by Crystal MRSA ID System.

Authors:  S M Qadri; Y Ueno; H Imambaccus; E Almodovar
Journal:  J Clin Microbiol       Date:  1994-07       Impact factor: 5.948
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  46 in total

1.  Detection of methicillin-resistant Staphylococcus aureus (MRSA) in blood with the EVIGENE MRSA detection kit.

Authors:  Katrina Levi; Kevin J Towner
Journal:  J Clin Microbiol       Date:  2003-08       Impact factor: 5.948

Review 2.  [The role of molecular genetics in dermatologic diagnosis].

Authors:  M Braun-Falco; T Ruzicka
Journal:  Hautarzt       Date:  2012-04       Impact factor: 0.751

3.  Value of PCR in surgically treated patients with staphylococcal infective endocarditis: a 4-year retrospective study.

Authors:  B Zaloudíková; E Němcová; J Pol; Z Sorm; S Wurmová; K Novotná; M Vaněrková; V Holá; F Růžička; L Dušek; P Němec; T Freiberger
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2011-10-02       Impact factor: 3.267

4.  Alternative use for spectra MRSA chromogenic agar in detection of methicillin-resistant Staphylococcus aureus from positive blood cultures.

Authors:  Jess F Peterson; Alexander A Dionisio; Katherine M Riebe; Gerri S Hall; Deborah A Wilson; Susan Whittier; Joseph R Dipersio; Nathan A Ledeboer
Journal:  J Clin Microbiol       Date:  2010-04-14       Impact factor: 5.948

5.  Panton-Valentine leukocidin-positive Staphylococcus aureus infections in returning travelers.

Authors:  Dennis Tappe; Marco H Schulze; Anett Oesterlein; Doris Turnwald; Andreas Müller; Ulrich Vogel; August Stich
Journal:  Am J Trop Med Hyg       Date:  2010-10       Impact factor: 2.345

Review 6.  Multidrug evolutionary strategies to reverse antibiotic resistance.

Authors:  Michael Baym; Laura K Stone; Roy Kishony
Journal:  Science       Date:  2016-01-01       Impact factor: 47.728

7.  Rapid detection of methicillin-resistant Staphylococcus aureus from screening enrichment broths by real-time PCR.

Authors:  K Levi; K J Towner
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2005-06       Impact factor: 3.267

8.  Verification of the IDI-MRSA assay for detecting methicillin-resistant Staphylococcus aureus in diverse specimen types in a core clinical laboratory setting.

Authors:  Steven J Drews; Barbara M Willey; Nathan Kreiswirth; Min Wang; Teresa Ianes; Jayne Mitchell; Mary Latchford; Allison J McGeer; Kevin C Katz
Journal:  J Clin Microbiol       Date:  2006-10       Impact factor: 5.948

9.  Design and validation of a low density array (Nosochip) for the detection and identification of the main pathogenic bacteria and fungi responsible for nosocomial pneumonia.

Authors:  S Burteau; P Bogaerts; R de Mendonça; L Irenge; C Berhin; J Hiffe; N de San; P Beyne; S Hamels; Y Glupczynski; M Struelens; J-L Gala; J Remacle
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2008-01       Impact factor: 3.267

10.  Comparison of culture screening methods for detection of nasal carriage of methicillin-resistant Staphylococcus aureus: a prospective study comparing 32 methods.

Authors:  Nasia Safdar; Leah Narans; Barbara Gordon; Dennis G Maki
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948
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