Literature DB >> 12882582

Real-time PCR method to detect Enterococcus faecalis in water.

Jorge W Santo Domingo1, Shawn C Siefring, Richard A Haugland.   

Abstract

A 16S rDNA real-time PCR method was developed to detect Enterococcus faecalis in water samples. The dynamic range for cell detection spanned five logs and the detection limit was determined to be 6 cfu/reaction. The assay was capable of detecting E. faecalis cells added to biofilms from a simulator of a water distribution system and in freshwater samples. Nucleic acid extraction was not required, permitting the detection of E. faecalis cells in less than 3 h.

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Year:  2003        PMID： 12882582     DOI： 10.1023/a:1022303118122

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  18 in total

1.  Development of Bacteroides 16S rRNA gene TaqMan-based real-time PCR assays for estimation of total, human, and bovine fecal pollution in water.

Authors:  Alice Layton; Larry McKay; Dan Williams; Victoria Garrett; Randall Gentry; Gary Sayler
Journal:  Appl Environ Microbiol       Date:  2006-06       Impact factor: 4.792

2.  Development and applications of microbial ecogenomic indicators for monitoring water quality: report of a workshop assessing the state of the science, research needs and future directions.

Authors:  Richard Devereux; Parke Rublee; John H Paul; Katharine G Field; Jorge W Santo Domingo
Journal:  Environ Monit Assess       Date:  2006-05       Impact factor: 2.513

3.  Quantitative detection of Listeria monocytogenes in biofilms by real-time PCR.

Authors:  Morgan Guilbaud; Pierre de Coppet; Fabrice Bourion; Cinta Rachman; Hervé Prévost; Xavier Dousset
Journal:  Appl Environ Microbiol       Date:  2005-04       Impact factor: 4.792

4.  Distribution of human-specific bacteroidales and fecal indicator bacteria in an urban watershed impacted by sewage pollution, determined using RNA- and DNA-based quantitative PCR assays.

Authors:  Vikram Kapoor; Tarja Pitkänen; Hodon Ryu; Michael Elk; David Wendell; Jorge W Santo Domingo
Journal:  Appl Environ Microbiol       Date:  2014-10-17       Impact factor: 4.792

5.  Intestinal microbiota and species diversity of Campylobacter and Helicobacter spp. in migrating shorebirds in Delaware Bay.

Authors:  Hodon Ryu; Kirsten Grond; Bram Verheijen; Michael Elk; Deborah M Buehler; Jorge W Santo Domingo
Journal:  Appl Environ Microbiol       Date:  2014-01-10       Impact factor: 4.792

6.  Rapid Identification of Vancomycin Resistant Enterococcus Faecalis Clinical Isolates using a Sugar Fermentation Method.

Authors:  Javad Raeisi; Mahnaz Saifi; Mohammad Reza Pourshafie; Mehri Habibi; Hamid Reza Mohajerani; Neda Akbari; Mohammad Reza Asadi Karam
Journal:  J Clin Diagn Res       Date:  2017-03-01

7.  Quantitative detection of Staphylococcus aureus and Enterococcus faecalis DNA in blood to diagnose bacteremia in patients in the intensive care unit.

Authors:  Remco P H Peters; Michiel A van Agtmael; Sonja Gierveld; Sven A Danner; A B Johan Groeneveld; Christina M J E Vandenbroucke-Grauls; Paul H M Savelkoul
Journal:  J Clin Microbiol       Date:  2007-09-19       Impact factor: 5.948

8.  23S rRNA gene-based enterococci community signatures in Lake Pontchartrain, Louisiana, USA, following urban runoff inputs after Hurricane Katrina.

Authors:  Hee-Sung Bae; Aixin Hou
Journal:  Microb Ecol       Date:  2012-12-28       Impact factor: 4.552

9.  Modification of Fecal Bacteria Counts and Blood Immune Cells in the Offspring of BALB/c and C57BL/6 Mice Obtained through Interstrain Mouse Embryo Transfer.

Authors:  Ekaterina A Litvinova; Galina V Kontsevaya; Elena N Kozhevnikova; Kseniya M Achasova; Ludmila A Gerlinskaya; Natalya A Feofanova; Mikhail P Moshkin
Journal:  J Am Assoc Lab Anim Sci       Date:  2020-06-09       Impact factor: 1.232

10.  Development of quantitative PCR assays targeting the 16S rRNA genes of Enterococcus spp. and their application to the identification of enterococcus species in environmental samples.

Authors:  Hodon Ryu; Michael Henson; Michael Elk; Carlos Toledo-Hernandez; John Griffith; Denene Blackwood; Rachel Noble; Michèle Gourmelon; Susan Glassmeyer; Jorge W Santo Domingo
Journal:  Appl Environ Microbiol       Date:  2012-10-19       Impact factor: 4.792
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