Literature DB >> 12839812

Rapid staining and enumeration of small numbers of total bacteria in water by solid-phase laser cytometry.

Susan C Broadaway1, Stephanie A Barton, Barry H Pyle.   

Abstract

The nucleic acid stain SYBR Green I was evaluated for use with solid-phase laser cytometry to obtain total bacterial cell counts from several water sources with small bacterial numbers. Results were obtained within 30 min and exceeded or equaled counts on R2A agar plates incubated for 14 days at room temperature.

Entities:  

Keywords:  NASA Discipline Cell Biology; NASA Discipline Life Support Systems; Non-NASA Center

Mesh：

Substances：

Year:  2003        PMID： 12839812      PMCID： PMC165144          DOI： 10.1128/AEM.69.7.4272-4273.2003

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  8 in total

Review 1.  Use of fluorescent probes to assess physiological functions of bacteria at single-cell level.

Authors:  F Joux; P Lebaron
Journal:  Microbes Infect       Date:  2000-10       Impact factor: 2.700

2.  Two and three-color fluorescence flow cytometric analysis of immunoidentified viable bacteria.

Authors:  S Barbesti; S Citterio; M Labra; M D Baroni; M G Neri; S Sgorbati
Journal:  Cytometry       Date:  2000-07-01

Review 3.  Current and future applications of flow cytometry in aquatic microbiology.

Authors:  J Vives-Rego; P Lebaron; G Nebe-von Caron
Journal:  FEMS Microbiol Rev       Date:  2000-10       Impact factor: 16.408

4.  Sensitive detection of Escherichia coli O157:H7 in food and water by immunomagnetic separation and solid-phase laser cytometry.

Authors:  B H Pyle; S C Broadaway; G A McFeters
Journal:  Appl Environ Microbiol       Date:  1999-05       Impact factor: 4.792

5.  Enumeration and Cell Cycle Analysis of Natural Populations of Marine Picoplankton by Flow Cytometry Using the Nucleic Acid Stain SYBR Green I.

Authors:  D Marie; F Partensky; S Jacquet; D Vaulot
Journal:  Appl Environ Microbiol       Date:  1997-01       Impact factor: 4.792

6.  Solid phase cytometry for detection of rare events.

Authors:  K Mignon-Godefroy; J G Guillet; C Butor
Journal:  Cytometry       Date:  1997-04-01

7.  Comparison of blue nucleic acid dyes for flow cytometric enumeration of bacteria in aquatic systems.

Authors:  P Lebaron; N Parthuisot; P Catala
Journal:  Appl Environ Microbiol       Date:  1998-05       Impact factor: 4.792

Review 8.  Use of fluorochromes for direct enumeration of total bacteria in environmental samples: past and present.

Authors:  R L Kepner; J R Pratt
Journal:  Microbiol Rev       Date:  1994-12
  8 in total
  6 in total

1.  Visualization of DNA-containing structures in various species of Chlorophyta, Rhodophyta and Cyanophyta using SYBR Green I dye.

Authors:  M Vítová; J Hendrychová; V Cepák; V Zachleder
Journal:  Folia Microbiol (Praha)       Date:  2005       Impact factor: 2.099

2.  Detection and quantification of viable airborne bacteria and fungi using solid-phase cytometry.

Authors:  Lies M E Vanhee; Hans J Nelis; Tom Coenye
Journal:  Nat Protoc       Date:  2009       Impact factor: 13.491

3.  Digital Holographic Microscopy, a Method for Detection of Microorganisms in Plume Samples from Enceladus and Other Icy Worlds.

Authors:  Manuel Bedrossian; Chris Lindensmith; Jay L Nadeau
Journal:  Astrobiology       Date:  2017-07-14       Impact factor: 4.335

4.  Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM).

Authors:  Manuel Bedrossian; Casey Barr; Chris A Lindensmith; Kenneth Nealson; Jay L Nadeau
Journal:  J Vis Exp       Date:  2017-11-01       Impact factor: 1.355

5.  Validating Flow Cytometry as a Method for Quantifying Bdellovibrio Predatory Bacteria and Its Prey for Microbial Ecology.

Authors:  Ayo Ogundero; Marta Vignola; Stephanie Connelly; William T Sloan
Journal:  Microbiol Spectr       Date:  2022-02-23

6.  Novel, rapid, and inexpensive cell-based quantification of antimalarial drug efficacy.

Authors:  Tyler N Bennett; Michelle Paguio; Bojana Gligorijevic; Clement Seudieu; Andrew D Kosar; Eugene Davidson; Paul D Roepe
Journal:  Antimicrob Agents Chemother       Date:  2004-05       Impact factor: 5.191
  6 in total

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