Literature DB >> 12791898

Comparison of washing and swabbing procedures for collecting genital fluids to assess cervicovaginal shedding of herpes simplex virus type 2 DNA.

Angélique Ndjoyi-Mbiguino1, Francis Ozouaki, Jérôme Legoff, François-Xavier Mbopi-Kéou, Ali Si-Mohamed, Isabelle Ndombi Onas, Evelyne Avoune, Laurent Bélec.   

Abstract

Asymptomatic genital shedding of herpes simplex virus type 2 (HSV-2) DNA was evidenced by real-time PCR in 25 (13.2%) of 188 cervicovaginal lavage samples and in only 13 (6.9%) paired cervicovaginal samples from 188 HSV-2-seropositive, nonpregnant childbearing-aged human immunodeficiency virus-seronegative women living in Gabon. These observations demonstrate that cervicovaginal washing is more suitable than endocervicovaginal swabbing for detecting and quantifying HSV-2 DNA by PCR in female genital secretions.

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Year:  2003        PMID： 12791898      PMCID： PMC156498          DOI： 10.1128/JCM.41.6.2662-2664.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

1.  A monoclonal blocking EIA for herpes simplex virus type 2 antibody: validation for seroepidemiological studies in Africa.

Authors:  R Gopal; T Gibbs; M J Slomka; J Whitworth; L M Carpenter; A Vyse; D W Brown
Journal:  J Virol Methods       Date:  2000-06       Impact factor: 2.014

2.  Detection of Herpes simplex virus DNA by real-time PCR.

Authors:  H H Kessler; G Mühlbauer; B Rinner; E Stelzl; A Berger; H W Dörr; B Santner; E Marth; H Rabenau
Journal:  J Clin Microbiol       Date:  2000-07       Impact factor: 5.948

3.  Polymerase chain reaction for Y chromosome to detect semen in cervicovaginal fluid: a prerequisite to assess HIV-specific vaginal immunity and HIV genital shedding.

Authors:  N Chomont; G Grésenguet; H Hocini; P Becquart; M Matta; L Andreoletti; A Si-Mohamed; M P Carreno; M Kazatchkine; L Bélec
Journal:  AIDS       Date:  2001-04-13       Impact factor: 4.177

4.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

Authors:  R K Saiki; S Scharf; F Faloona; K B Mullis; G T Horn; H A Erlich; N Arnheim
Journal:  Science       Date:  1985-12-20       Impact factor: 47.728

5.  Genital herpes and public health: addressing a global problem.

Authors:  L Corey; H H Handsfield
Journal:  JAMA       Date:  2000-02-09       Impact factor: 56.272

Review 6.  From epidemiological synergy to public health policy and practice: the contribution of other sexually transmitted diseases to sexual transmission of HIV infection.

Authors:  D T Fleming; J N Wasserheit
Journal:  Sex Transm Infect       Date:  1999-02       Impact factor: 3.519

7.  Interactions between herpes simplex virus type 2 and human immunodeficiency virus type 1 infection in African women: opportunities for intervention.

Authors:  F X Mbopi-Kéou; G Grésenguet; P Mayaud; H A Weiss; R Gopal; M Matta; J L Paul; D W Brown; R J Hayes; D C Mabey; L Bélec
Journal:  J Infect Dis       Date:  2000-09-08       Impact factor: 5.226

8.  Selection of drug-resistant variants in the female genital tract of human immunodeficiency virus type 1-infected women receiving antiretroviral therapy.

Authors:  A Si-Mohamed; M D Kazatchkine; I Heard; C Goujon; T Prazuck; G Aymard; G Cessot; Y H Kuo; M C Bernard; B Diquet; J E Malkin; L Gutmann; L Bélec
Journal:  J Infect Dis       Date:  2000-07-06       Impact factor: 5.226

9.  Detection and quantitation of human immunodeficiency virus type 1 in the female genital tract. The Division of AIDS Treatment Research Initiative 009 Study Group.

Authors:  P Baron; J Bremer; S S Wasserman; M Nowicki; B Driscoll; B Polsky; A Kovacs; P S Reichelderfer
Journal:  J Clin Microbiol       Date:  2000-10       Impact factor: 5.948

10.  Dilution assessment of cervicovaginal secretions obtained by vaginal washing for immunological assays.

Authors:  L Bélec; D Meillet; M Lévy; A Georges; C Tévi-Bénissan; J Pillot
Journal:  Clin Diagn Lab Immunol       Date:  1995-01
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  5 in total

1.  Real-time PCR quantification of genital shedding of herpes simplex virus (HSV) and human immunodeficiency virus (HIV) in women coinfected with HSV and HIV.

Authors:  Jérôme Legoff; Hicham Bouhlal; Gérard Grésenguet; Helen Weiss; Nzambi Khonde; Hakim Hocini; Nathalie Désiré; Ali Si-Mohamed; Jean de Dieu Longo; Cécile Chemin; Eric Frost; Jacques Pépin; Jean-Elie Malkin; Philippe Mayaud; Laurent Bélec
Journal:  J Clin Microbiol       Date:  2006-02       Impact factor: 5.948

2.  Comparison of sampling methods to measure HIV RNA viral load in female genital tract secretions.

Authors:  Shameem Z Jaumdally; Heidi E Jones; Donald R Hoover; Hoyam Gamieldien; Jean-Mari Kriek; Nontokozo Langwenya; Landon Myer; Jo-Ann S Passmore; Catherine S Todd
Journal:  Am J Reprod Immunol       Date:  2017-01-23       Impact factor: 3.886

3.  Rapid diagnosis of herpes simplex virus infection by a loop-mediated isothermal amplification method.

Authors:  Yoshihiko Enomoto; Tetsushi Yoshikawa; Masaru Ihira; Shiho Akimoto; Fumi Miyake; Chie Usui; Sadao Suga; Kayoko Suzuki; Takashi Kawana; Yukihiro Nishiyama; Yoshizo Asano
Journal:  J Clin Microbiol       Date:  2005-02       Impact factor: 5.948

4.  Reconsideration of viral protein immunoblotting for differentiation of human herpes simplex viruses.

Authors:  Robert N Bowles; Jamie C Yedowitz; John A Blaho
Journal:  Diagn Microbiol Infect Dis       Date:  2008-07-17       Impact factor: 2.803

5.  Changes in plasma viral load and penile viral shedding after circumcision among HIV-positive men in Kisumu, Kenya.

Authors:  Elijah Odoyo-June; John H Rogers; Walter Jaoko; Robert C Bailey
Journal:  J Acquir Immune Defic Syndr       Date:  2013-12-15       Impact factor: 3.731
  5 in total

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