Literature DB >> 12777696

Automated pneumococcal MLST using liquid-handling robotics and a capillary DNA sequencer.

Johanna Jefferies1, Stuart C Clarke, Mathew A Diggle, Andrew Smith, Chris Dowson, Tim Mitchell.   

Abstract

Multilocus sequence typing (MLST) is used by the Scottish Meningococcus and Pneumococcus Reference Laboratory (SMPRL) as a routine method for the characterization of certain bacterial pathogens. The SMPRL recently started performing MLST on strains of Streptococcus pneumoniae, and here we describe a fully automated method for MLST using a 96-well-format liquid-handling robot and a 96-capillary automated DNA sequencer.

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Year:  2003        PMID： 12777696     DOI： 10.1385/MB:24:3:303

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.860


  9 in total

1.  Rapid assignment of nucleotide sequence data to allele types for multi-locus sequence analysis (MLSA) of bacteria using an adapted database and modified alignment program.

Authors:  M A Diggle; S C Clarke
Journal:  J Mol Microbiol Biotechnol       Date:  2002-11

2.  Automated PCR/sequence template purification.

Authors:  S C Clarke; M A Diggle
Journal:  Mol Biotechnol       Date:  2002-07       Impact factor: 2.695

3.  Pneumococcal serotypes and their clinical relevance.

Authors:  M Kalin
Journal:  Thorax       Date:  1998-03       Impact factor: 9.139

4.  Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms.

Authors:  M C Maiden; J A Bygraves; E Feil; G Morelli; J E Russell; R Urwin; Q Zhang; J Zhou; K Zurth; D A Caugant; I M Feavers; M Achtman; B G Spratt
Journal:  Proc Natl Acad Sci U S A       Date:  1998-03-17       Impact factor: 11.205

5.  Serotyping of Streptococcus pneumoniae strains by coagglutination.

Authors:  L E Smart
Journal:  J Clin Pathol       Date:  1986-03       Impact factor: 3.411

6.  Serogroup-specific epidemiology of Streptococcus pneumoniae: associations with age, sex, and geography in 7,000 episodes of invasive disease.

Authors:  J A Scott; A J Hall; R Dagan; J M Dixon; S J Eykyn; A Fenoll; M Hortal; L P Jetté; J H Jorgensen; F Lamothe; C Latorre; J T Macfarlane; D M Shlaes; L E Smart; A Taunay
Journal:  Clin Infect Dis       Date:  1996-06       Impact factor: 9.079

7.  Semiautomation of multilocus sequence typing for the characterization of clinical isolates of Neisseria meningitidis.

Authors:  S C Clarke; M A Diggle; G F Edwards
Journal:  J Clin Microbiol       Date:  2001-09       Impact factor: 5.948

8.  A multilocus sequence typing scheme for Streptococcus pneumoniae: identification of clones associated with serious invasive disease.

Authors:  Mark C Enright; Brian G Spratt
Journal:  Microbiology (Reading)       Date:  1998-11       Impact factor: 2.777

9.  An alternative approach to typing of Streptococcus pneumoniae strains by coagglutination.

Authors:  L E Smart; J Henrichsen
Journal:  Acta Pathol Microbiol Immunol Scand B       Date:  1986-12
  9 in total
  13 in total

1.  Serotypes and sequence types of pneumococci causing invasive disease in Scotland prior to the introduction of pneumococcal conjugate polysaccharide vaccines.

Authors:  S C Clarke; K J Scott; S M McChlery
Journal:  J Clin Microbiol       Date:  2004-10       Impact factor: 5.948

Review 2.  Multilocus sequence typing: Data analysis in clinical microbiology and public health.

Authors:  Christopher B Sullivan; Matthew A Diggle; Stuart C Clarke
Journal:  Mol Biotechnol       Date:  2005-03       Impact factor: 2.695

3.  Automation of MLST using third-generation liquid-handling technology.

Authors:  C B Sullivan; J M C Jefferies; M A Diggle; S C Clarke
Journal:  Mol Biotechnol       Date:  2006-03       Impact factor: 2.695

Review 4.  Microbial sequence typing in the genomic era.

Authors:  Marcos Pérez-Losada; Miguel Arenas; Eduardo Castro-Nallar
Journal:  Infect Genet Evol       Date:  2017-09-21       Impact factor: 3.342

5.  Clonal analysis of invasive pneumococcal isolates in Scotland and coverage of serotypes by the licensed conjugate polysaccharide pneumococcal vaccine: possible implications for UK vaccine policy.

Authors:  S M McChlery; K J Scott; S C Clarke
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2005-04       Impact factor: 3.267

6.  Molecular epidemiology of pneumococcal colonization in response to pneumococcal conjugate vaccination in children with recurrent acute otitis media.

Authors:  D Bogaert; R H Veenhoven; M Sluijter; W J W Wannet; G T Rijkers; T J Mitchell; S C Clarke; W H F Goessens; A G Schilder; E A M Sanders; R de Groot; P W M Hermans
Journal:  J Clin Microbiol       Date:  2005-01       Impact factor: 5.948

7.  Genetic analysis of diverse disease-causing pneumococci indicates high levels of diversity within serotypes and capsule switching.

Authors:  Johanna M C Jefferies; Andrew Smith; Stuart C Clarke; Chris Dowson; Timothy J Mitchell
Journal:  J Clin Microbiol       Date:  2004-12       Impact factor: 5.948

8.  Molecular epidemiology of penicillin-susceptible non-beta-lactam-resistant Streptococcus pneumoniae isolates from Greek children.

Authors:  D Bogaert; P W M Hermans; I N Grivea; G S Katopodis; T J Mitchell; M Sluijter; R De Groot; N G Beratis; G A Syrogiannopoulos
Journal:  J Clin Microbiol       Date:  2003-12       Impact factor: 5.948

9.  Multilocus sequence typing.

Authors:  Ana Belén Ibarz Pavón; Martin C J Maiden
Journal:  Methods Mol Biol       Date:  2009

10.  Simultaneous nasopharyngeal carriage of two pneumococcal multilocus sequence types with a serotype 3 phenotype.

Authors:  Donald Inverarity; Mathew Diggle; Roisin Ure; Diego Santana-Hernandez; Peter Altstadt; Timothy Mitchell; Giles Edwards
Journal:  Int J Microbiol       Date:  2010-12-01
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