Literature DB >> 16632888

Automation of MLST using third-generation liquid-handling technology.

C B Sullivan1, J M C Jefferies, M A Diggle, S C Clarke.   

Abstract

The molecular characterization of bacterial pathogens of clinical significance is increasingly important. Methods, such as multilocus sequence typing (MLST), allow bacterial strains to be characterized during case clusters, for antibiotic-resistant strains to be monitored, and for the impact of new vaccines to be assessed. Our laboratory performs MLST on Neisseria meningitidis, Streptococcus pneumoniae, Haemophilus influenzae, and Staphylococcus aureus. We have developed high-throughput automated methods to allow MLST to be performed in a time scale useful in a clinical setting. Here we describe the automation of MLST on a third-generation liquid-handling robot.

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Year:  2006        PMID： 16632888     DOI： 10.1385/MB:32:3:219

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  13 in total

1.  Characterization of encapsulated and noncapsulated Haemophilus influenzae and determination of phylogenetic relationships by multilocus sequence typing.

Authors:  Emma Meats; Edward J Feil; Suzanna Stringer; Alison J Cody; Richard Goldstein; J Simon Kroll; Tanja Popovic; Brian G Spratt
Journal:  J Clin Microbiol       Date:  2003-04       Impact factor: 5.948

2.  Multilocus sequence typing and porA gene sequencing differentiates strains of Neisseria meningitidis during case clusters.

Authors:  S C Clarke; M A Diggle; G F S Edwards
Journal:  Br J Biomed Sci       Date:  2002       Impact factor: 3.829

3.  Rapid assignment of nucleotide sequence data to allele types for multi-locus sequence analysis (MLSA) of bacteria using an adapted database and modified alignment program.

Authors:  M A Diggle; S C Clarke
Journal:  J Mol Microbiol Biotechnol       Date:  2002-11

Review 4.  Nucleotide sequence-based typing of bacteria and the impact of automation.

Authors:  Stuart C Clarke
Journal:  Bioessays       Date:  2002-09       Impact factor: 4.345

5.  Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms.

Authors:  M C Maiden; J A Bygraves; E Feil; G Morelli; J E Russell; R Urwin; Q Zhang; J Zhou; K Zurth; D A Caugant; I M Feavers; M Achtman; B G Spratt
Journal:  Proc Natl Acad Sci U S A       Date:  1998-03-17       Impact factor: 11.205

6.  Semiautomation of multilocus sequence typing for the characterization of clinical isolates of Neisseria meningitidis.

Authors:  S C Clarke; M A Diggle; G F Edwards
Journal:  J Clin Microbiol       Date:  2001-09       Impact factor: 5.948

7.  Introduction of an automated service for the laboratory confirmation of meningococcal disease in Scotland.

Authors:  S C Clarke; M A Diggle; J A Reid; L Thom; G F Edwards
Journal:  J Clin Pathol       Date:  2001-07       Impact factor: 3.411

8.  A multilocus sequence typing scheme for Streptococcus pneumoniae: identification of clones associated with serious invasive disease.

Authors:  Mark C Enright; Brian G Spratt
Journal:  Microbiology (Reading)       Date:  1998-11       Impact factor: 2.777

9.  Population genetic and evolutionary approaches to analysis of Neisseria meningitidis isolates belonging to the ET-5 complex.

Authors:  J A Bygraves; R Urwin; A J Fox; S J Gray; J E Russell; I M Feavers; M C Maiden
Journal:  J Bacteriol       Date:  1999-09       Impact factor: 3.490

10.  Automated pneumococcal MLST using liquid-handling robotics and a capillary DNA sequencer.

Authors:  Johanna Jefferies; Stuart C Clarke; Mathew A Diggle; Andrew Smith; Chris Dowson; Tim Mitchell
Journal:  Mol Biotechnol       Date:  2003-07       Impact factor: 2.860
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  12 in total

Review 1.  Microbial sequence typing in the genomic era.

Authors:  Marcos Pérez-Losada; Miguel Arenas; Eduardo Castro-Nallar
Journal:  Infect Genet Evol       Date:  2017-09-21       Impact factor: 3.342

2.  Multicenter study for defining the breakpoint for rifampin resistance in Neisseria meningitidis by rpoB sequencing.

Authors:  Muhamed-Kheir Taha; Sara Thulin Hedberg; Marek Szatanik; Eva Hong; Corinne Ruckly; Raquel Abad; Sophie Bertrand; Francoise Carion; Heike Claus; Alejandra Corso; Rocío Enríquez; Sigrid Heuberger; Waleria Hryniewicz; Keith A Jolley; Paula Kriz; Marta Mollerach; Martin Musilek; Arianna Neri; Per Olcén; Marina Pana; Anna Skoczynska; Cecilia Sorhouet Pereira; Paola Stefanelli; Georgina Tzanakaki; Magnus Unemo; Julio A Vázquez; Ulrich Vogel; Izabela Wasko
Journal:  Antimicrob Agents Chemother       Date:  2010-07-06       Impact factor: 5.191

3.  Temporal analysis of invasive pneumococcal clones from Scotland illustrates fluctuations in diversity of serotype and genotype in the absence of pneumococcal conjugate vaccine.

Authors:  J M Jefferies; A J Smith; G F S Edwards; J McMenamin; T J Mitchell; S C Clarke
Journal:  J Clin Microbiol       Date:  2009-11-18       Impact factor: 5.948

4.  Target gene sequencing to characterize the penicillin G susceptibility of Neisseria meningitidis.

Authors:  Muhamed-Kheir Taha; Julio A Vázquez; Eva Hong; Desiree E Bennett; Sophie Bertrand; Suzana Bukovski; Mary T Cafferkey; Françoise Carion; Jens Jørgen Christensen; Mathew Diggle; Giles Edwards; Rocío Enríquez; Cecilia Fazio; Matthias Frosch; Sigrid Heuberger; Steen Hoffmann; Keith A Jolley; Marcin Kadlubowski; Amel Kechrid; Konstantinos Kesanopoulos; Paula Kriz; Lotte Lambertsen; Ileanna Levenet; Martin Musilek; Metka Paragi; Aouatef Saguer; Anna Skoczynska; Paola Stefanelli; Sara Thulin; Georgina Tzanakaki; Magnus Unemo; Ulrich Vogel; Maria Leticia Zarantonelli
Journal:  Antimicrob Agents Chemother       Date:  2007-05-21       Impact factor: 5.191

Review 5.  Automated extraction of typing information for bacterial pathogens from whole genome sequence data: Neisseria meningitidis as an exemplar.

Authors:  K A Jolley; M C Maiden
Journal:  Euro Surveill       Date:  2013-01-24

6.  Transforming microbial genotyping: a robotic pipeline for genotyping bacterial strains.

Authors:  Brian O'Farrell; Jana K Haase; Vimalkumar Velayudhan; Ronan A Murphy; Mark Achtman
Journal:  PLoS One       Date:  2012-10-29       Impact factor: 3.240

7.  Simultaneous nasopharyngeal carriage of two pneumococcal multilocus sequence types with a serotype 3 phenotype.

Authors:  Donald Inverarity; Mathew Diggle; Roisin Ure; Diego Santana-Hernandez; Peter Altstadt; Timothy Mitchell; Giles Edwards
Journal:  Int J Microbiol       Date:  2010-12-01

8.  Death or survival from invasive pneumococcal disease in Scotland: associations with serogroups and multilocus sequence types.

Authors:  Donald Inverarity; Karen Lamb; Mathew Diggle; Chris Robertson; David Greenhalgh; Tim J Mitchell; Andrew Smith; Johanna M C Jefferies; Stuart C Clarke; Jim McMenamin; Giles F S Edwards
Journal:  J Med Microbiol       Date:  2011-03-10       Impact factor: 2.472

9.  Clonal analysis of meningococci during a 26 year period prior to the introduction of meningococcal serogroup C vaccines.

Authors:  Christopher B Sullivan; Mathew A Diggle; Robert L Davies; Stuart C Clarke
Journal:  PLoS One       Date:  2015-01-23       Impact factor: 3.240

10.  Evolutionary Events Associated with an Outbreak of Meningococcal Disease in Men Who Have Sex with Men.

Authors:  Muhamed-Kheir Taha; Heike Claus; Martin Lappann; Frédéric J Veyrier; Andreas Otto; Dörte Becher; Ala-Eddine Deghmane; Matthias Frosch; Wiebke Hellenbrand; Eva Hong; Isabelle Parent du Châtelet; Karola Prior; Dag Harmsen; Ulrich Vogel
Journal:  PLoS One       Date:  2016-05-11       Impact factor: 3.240
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