| Literature DB >> 12740605 |
Sophie Desloire1, Hassen Gherbi, Wassila Laloui, Sylvie Marhadour, Vanessa Clouet, Laurence Cattolico, Cyril Falentin, Sandra Giancola, Michel Renard, Françoise Budar, Ian Small, Michel Caboche, Régine Delourme, Abdelhafid Bendahmane.
Abstract
Ogura cytoplasmic male sterility (CMS) in radish (Raphanus sativus) is caused by an aberrant mitochondrial gene, Orf138, that prevents the production of functional pollen without affecting female fertility. Rfo, a nuclear gene that restores male fertility, alters the expression of Orf138 at the post-transcriptional level. The Ogura CMS/Rfo two-component system is a useful model for investigating nuclear-cytoplasmic interactions, as well as the physiological basis of fertility restoration. Using a combination of positional cloning and microsynteny analysis of Arabidopsis thaliana and radish, we genetically and physically delimited the Rfo locus to a 15-kb DNA segment. Analysis of this segment shows that Rfo is a member of the pentatricopeptide repeat (PPR) family. In Arabidopsis, this family contains more than 450 members of unknown function, although most of them are predicted to be targeted to mitochondria and chloroplasts and are thought to have roles in organellar gene expression.Entities:
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Year: 2003 PMID： 12740605 PMCID： PMC1319198 DOI： 10.1038/sj.embor.embor848
Source DB: PubMed Journal: EMBO Rep ISSN： 1469-221X Impact factor: 8.807