| Literature DB >> 12732581 |
Masayo Date1, Kei-ichi Yokoyama, Yukiko Umezawa, Hiroshi Matsui, Yoshimi Kikuchi.
Abstract
We previously observed secretion of active-form transglutaminase in Corynebacterium glutamicum by coexpressing the subtilisin-like protease SAM-P45 from Streptomyces albogriseolus to process the prodomain. However, the N-terminal amino acid sequence of the transglutaminase differed from that of the native Streptoverticillium mobaraense enzyme. In the present work we have used site-directed mutagenesis to generate an optimal SAM-P45 cleavage site in the C-terminal region of the prodomain. As a result, native-type transglutaminase was secreted.Entities:
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Year: 2003 PMID: 12732581 PMCID: PMC154552 DOI: 10.1128/AEM.69.5.3011-3014.2003
Source DB: PubMed Journal: Appl Environ Microbiol ISSN: 0099-2240 Impact factor: 4.792