| Literature DB >> 12676982 |
H Yang1, P D Carr, S Yu McLoughlin, J W Liu, I Horne, X Qiu, C M J Jeffries, R J Russell, J G Oakeshott, D L Ollis.
Abstract
Organophosphate-degrading enzyme from Agrobacterium radiobacter P230 (OPDA) is a recently discovered enzyme that degrades a broad range of organophosphates. It is very similar to OPH first isolated from Pseudomonas diminuta MG. Despite a high level of sequence identity, OPH and OPDA exhibit different substrate specificities. We report here the structure of OPDA and identify regions of the protein that are likely to give it a preference for substrates that have shorter alkyl substituents. Directed evolution was used to evolve a series of OPH mutants that had activities similar to those of OPDA. Mutants were selected for on the basis of their ability to degrade a number of substrates. The mutations tended to cluster in particular regions of the protein and in most cases, these regions were where OPH and OPDA had significant differences in their sequences.Entities:
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Year: 2003 PMID： 12676982 DOI： 10.1093/proeng/gzg013
Source DB: PubMed Journal: Protein Eng ISSN： 0269-2139