Literature DB >> 12676725

Improving the thermostability of raw-starch-digesting amylase from a Cytophaga sp. by site-directed mutagenesis.

Rong-Jen Shiau1, Hui-Chen Hung, Chii-Ling Jeang.   

Abstract

A heat-stable raw-starch-digesting amylase (RSDA) was generated through PCR-based site-directed mutagenesis. At 65 degrees C, the half-life of this mutant RSDA, which, compared with the wild-type RSDA, lacks amino acids R178 and G179, was increased 20-fold. While the wild type was inactivated completely at pH 3.0, the mutant RSDA still retained 41% of its enzymatic activity. The enhancement of RSDA thermostability was demonstrated to be via a Ca(2+)-independent mechanism.

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Year:  2003        PMID： 12676725      PMCID： PMC154812          DOI： 10.1128/AEM.69.4.2383-2385.2003

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  19 in total

Review 1.  Protein engineering of bacterial alpha-amylases.

Authors:  J E Nielsen; T V Borchert
Journal:  Biochim Biophys Acta       Date:  2000-12-29

2.  Comparative characterization of complete and truncated forms of Lactobacillus amylovorus alpha-amylase and role of the C-terminal direct repeats in raw-starch binding.

Authors:  R Rodriguez Sanoja; J Morlon-Guyot; J Jore; J Pintado; N Juge; J P Guyot
Journal:  Appl Environ Microbiol       Date:  2000-08       Impact factor: 4.792

3.  Purification and properties of a novel raw starch degrading-cyclodextrin glycosyltransferase from Klebsiella pneumoniae AS- 22.

Authors:  B N. Gawande; A Y. Patkar
Journal:  Enzyme Microb Technol       Date:  2001-06-07       Impact factor: 3.493

4.  Nucleotide sequence of the Bacillus stearothermophilus alpha-amylase gene.

Authors:  R Nakajima; T Imanaka; S Aiba
Journal:  J Bacteriol       Date:  1985-07       Impact factor: 3.490

5.  Crystal structure of Bacillus stearothermophilus alpha-amylase: possible factors determining the thermostability.

Authors:  D Suvd; Z Fujimoto; K Takase; M Matsumura; H Mizuno
Journal:  J Biochem       Date:  2001-03       Impact factor: 3.387

6.  Amino acid residues stabilizing a Bacillus alpha-amylase against irreversible thermoinactivation.

Authors:  Y Suzuki; N Ito; T Yuuki; H Yamagata; S Udaka
Journal:  J Biol Chem       Date:  1989-11-15       Impact factor: 5.157

7.  Complete nucleotide sequence of a gene coding for heat- and pH-stable alpha-amylase of Bacillus licheniformis: comparison of the amino acid sequences of three bacterial liquefying alpha-amylases deduced from the DNA sequences.

Authors:  T Yuuki; T Nomura; H Tezuka; A Tsuboi; H Yamagata; N Tsukagoshi; S Udaka
Journal:  J Biochem       Date:  1985-11       Impact factor: 3.387

8.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

9.  Purification and characterization of a raw-starch digesting amylase from a soil bacterium--Cytophaga sp.

Authors:  C L Jeang; Y H Lee; L W Chang
Journal:  Biochem Mol Biol Int       Date:  1995-03

10.  Improved thermostability of a Bacillus alpha-amylase by deletion of an arginine-glycine residue is caused by enhanced calcium binding.

Authors:  K Igarashi; Y Hatada; K Ikawa; H Araki; T Ozawa; T Kobayashi; K Ozaki; S Ito
Journal:  Biochem Biophys Res Commun       Date:  1998-07-20       Impact factor: 3.575
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  4 in total

1.  SORS: a universal one-round PCR-based method for site-directed mutagenesis.

Authors:  Bao-Li Wang; Jin-Xing Quan; Hui Liang; Yan-Li Jiao; Xiao-Xia Li; Gang Guo; Jing-Yu Zhang
Journal:  Mol Biotechnol       Date:  2008-04-16       Impact factor: 2.695

2.  Stabilization of a raw starch digesting amylase from Aspergillus carbonarius via immobilization on activated and non-activated agarose gel.

Authors:  Tochukwu N Nwagu; Bartho N Okolo; Hideki Aoyagi
Journal:  World J Microbiol Biotechnol       Date:  2011-06-28       Impact factor: 3.312

3.  Close relationship of a novel Flavobacteriaceae α-amylase with archaeal α-amylases and good potentials for industrial applications.

Authors:  Chunfang Li; Miaofen Du; Bin Cheng; Lushan Wang; Xinqiang Liu; Cuiqing Ma; Chunyu Yang; Ping Xu
Journal:  Biotechnol Biofuels       Date:  2014-01-31       Impact factor: 6.040

Review 4.  The Importance of Surface-Binding Site towards Starch-Adsorptivity Level in α-Amylase: A Review on Structural Point of View.

Authors:  Umi Baroroh; Muhammad Yusuf; Saadah Diana Rachman; Safri Ishmayana; Mas Rizky A A Syamsunarno; Jutti Levita; Toto Subroto
Journal:  Enzyme Res       Date:  2017-12-05
  4 in total

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