Literature DB >> 12654855

Mucosal immunization with a genetically engineered pertussis toxin S1 fragment-cholera toxin subunit B chimeric protein.

Song F Lee1, Scott A Halperin, Danny F Salloum, Ann MacMillan, Annette Morris.   

Abstract

A chimeric protein consisting of a divalent pertussis toxin (PT) S1 fragment linked to the cholera toxin (Ctx) A(2)B fragment was constructed. The chimera induced a mucosal immunoglobulin A (IgA) and a serum IgG immune response to PT and CtxB in BALB/c mice following intranasal immunization. The immune sera neutralized PT in vitro. In the mouse model of Bordetella pertussis respiratory infection, the chimera-immunized animals showed a significant reduction in bacterial lung counts (P = 0.01) from that of the sham control group. Thus, a divalent S1 fragment CtxA2B chimera is an immunogenic antigen and can elicit a protective immunity.

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Year:  2003        PMID： 12654855      PMCID： PMC152103          DOI： 10.1128/IAI.71.4.2272-2275.2003

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  18 in total

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  20 in total

1.  Expression of a C terminally truncated form of pertussis toxin S1 subunit effectively induces protection against pertussis toxin following DNA-based immunization.

Authors:  Kazunari Kamachi; Yoshichika Arakawa
Journal:  Infect Immun       Date:  2004-07       Impact factor: 3.441

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Journal:  Infect Immun       Date:  2005-06       Impact factor: 3.441

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Authors:  Zhang Jinyong; Zhang Xiaoli; Zhang Weijun; Guo Ying; Guo Gang; Mao Xuhu; Zou Quanming
Journal:  Mol Biol Rep       Date:  2010-09-28       Impact factor: 2.316

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Journal:  Clin Vaccine Immunol       Date:  2011-07-06

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Journal:  Clin Vaccine Immunol       Date:  2007-08-22

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Authors:  Chiang W Lee; Song F Lee; Scott A Halperin
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Journal:  Infect Immun       Date:  2007-08-27       Impact factor: 3.441
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