| Literature DB >> 12615427 |
Alberto Fachado1, Alexandro Rodriguez, Sergio O Angel, Diamar C Pinto, Iviane Vila, Armando Acosta, Regina R Amendoeira, Joseli Lannes-Vieira.
Abstract
BALB/c mice were intramuscularly immunized with low doses (25-50microg) of DNA cocktail containing plasmids encoding the full-length SAG1/P30 and the 196-561 terminal sequence of ROP2 genes. This immunization resulted in a Th1-type response with predominance of IgG2a and a specific T-cell proliferation with high levels of interferon-gamma (IFN-gamma) secretion, whereas no IL-4 was detected. Moreover, DNA cocktail immunization induced a long-lasting protection against a lethal challenge with the highly virulent Toxoplasma gondii RH strain, whereas low doses of single genes were not protective. These results support further investigations to achieve a multigene anti-T. gondii DNA vaccine.Entities:
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Year: 2003 PMID： 12615427 DOI： 10.1016/s0264-410x(02)00692-8
Source DB: PubMed Journal: Vaccine ISSN： 0264-410X Impact factor: 3.641