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Literature DB >> 11414321

Efficient transformation of filamentous fungus Pleurotus ostreatus using single-strand carrier DNA.

T Irie¹, Y Honda, T Watanabe, M Kuwahara.

Abstract

The effects of carrier DNAs on the transformation of the basidiomycete Pleurotus ostreatus were analyzed. When lambda phage DNA was added to a transformation mixture containing protoplasts and CbxR vector plasmid, an increased number of drug-resistant transformants was observed on a screening plate containing 2 microg carboxin/ml. The highest efficiency (about 200 transformants/microg vector plasmid) was obtained by the addition of heat-denatured lambda DNA, which gave yields approximately 50-fold higher than the control experiment without a carrier DNA. To our knowledge, this is the first report on enhancement in transformation efficiency of fungal protoplasts by single strand carrier DNA.

Entities: Disease Species

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Year: 2001 PMID： 11414321 DOI： 10.1007/s002530000535

Source DB: PubMed Journal: Appl Microbiol Biotechnol ISSN： 0175-7598 Impact factor: 4.813

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Cited

14 in total

1. Effects of calmodulin on expression of lignin-modifying enzymes in Pleurotus ostreatus.

Authors: Takashi Suetomi; Takaiku Sakamoto; Yoshitaka Tokunaga; Toru Kameyama; Yoichi Honda; Hisatoshi Kamitsuji; Isamu Kameshita; Kousuke Izumitsu; Kazumi Suzuki; Toshikazu Irie
Journal: Curr Genet Date: 2014-11-19 Impact factor: 3.886

2. Predominance of a versatile-peroxidase-encoding gene, mnp4, as demonstrated by gene replacement via a gene targeting system for Pleurotus ostreatus.

Authors: Tomer M Salame; Doriv Knop; Dana Tal; Dana Levinson; Oded Yarden; Yitzhak Hadar
Journal: Appl Environ Microbiol Date: 2012-05-25 Impact factor: 4.792

3. Development of a transformation system for Crinipellis perniciosa, the causal agent of witches' broom in cocoa plants.

Authors: Juliana Oliveira Lima; Jildete Karla dos Santos; Jorge Fernando Pereira; Mário Lúcio Vilela de Resende; Elza Fernandes de Araújo; Marisa Vieira de Queiroz
Journal: Curr Genet Date: 2002-12-17 Impact factor: 3.886

10. Release of Pleurotus ostreatus versatile-peroxidase from Mn2+ repression enhances anthropogenic and natural substrate degradation.

Authors: Tomer M Salame; Doriv Knop; Dana Levinson; Sameer J Mabjeesh; Oded Yarden; Yitzhak Hadar
Journal: PLoS One Date: 2012-12-21 Impact factor: 3.240

Efficient transformation of filamentous fungus Pleurotus ostreatus using single-strand carrier DNA.

1. Effects of calmodulin on expression of lignin-modifying enzymes in Pleurotus ostreatus.

2. Predominance of a versatile-peroxidase-encoding gene, mnp4, as demonstrated by gene replacement via a gene targeting system for Pleurotus ostreatus.

3. Development of a transformation system for Crinipellis perniciosa, the causal agent of witches' broom in cocoa plants.

4. Redundancy among manganese peroxidases in Pleurotus ostreatus.

5. Agrobacterium tumefaciens-mediated genetic transformation of the phytopathogenic fungus Penicillium digitatum.

6. Quantification of M13 and T7 bacteriophages by TaqMan and SYBR green qPCR.

7. Pleurotus ostreatus manganese-dependent peroxidase silencing impairs decolourization of Orange II.

8. Identification and functional analysis of pheromone and receptor genes in the B3 mating locus of Pleurotus eryngii.

Review 9. Current technologies and related issues for mushroom transformation.

10. Release of Pleurotus ostreatus versatile-peroxidase from Mn2+ repression enhances anthropogenic and natural substrate degradation.