Literature DB >> 15956428

Use of the genomic subtractive hybridization technique to develop a real-time PCR assay for quantitative detection of Prevotella spp. in oral biofilm samples.

Shiori Nagashima1, Akihiro Yoshida, Nao Suzuki, Toshihiro Ansai, Tadamichi Takehara.   

Abstract

Genomic subtractive hybridization was used to design Prevotella nigrescens-specific primers and TaqMan probes. Based on this technique, a TaqMan real-time PCR assay was developed for quantifying four oral black-pigmented Prevotella species. The combination of real-time PCR and genomic subtractive hybridization is useful for preparing species-specific primer-probe sets for closely related species.

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Year:  2005        PMID： 15956428      PMCID： PMC1151961          DOI： 10.1128/JCM.43.6.2948-2951.2005

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  22 in total

1.  Isolation, cloning, and expression of an acid phosphatase containing phosphotyrosyl phosphatase activity from Prevotella intermedia.

Authors:  X Chen; T Ansai; S Awano; T Iida; S Barik; T Takehara
Journal:  J Bacteriol       Date:  1999-11       Impact factor: 3.490

2.  Purification and characterization of alkaline phosphatase containing phosphotyrosyl phosphatase activity from the bacterium Prevotella intermedia.

Authors:  T Ansai; S Awano; X Chen; T Fuchi; T Arimoto; S Akifusa; T Takehara
Journal:  FEBS Lett       Date:  1998-05-29       Impact factor: 4.124

3.  Real time quantitative PCR.

Authors:  C A Heid; J Stevens; K J Livak; P M Williams
Journal:  Genome Res       Date:  1996-10       Impact factor: 9.043

Review 4.  Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.

Authors:  S F Altschul; T L Madden; A A Schäffer; J Zhang; Z Zhang; W Miller; D J Lipman
Journal:  Nucleic Acids Res       Date:  1997-09-01       Impact factor: 16.971

5.  Cloning and characterization of a Prevotella melaninogenica hemolysin.

Authors:  H E Allison; J D Hillman
Journal:  Infect Immun       Date:  1997-07       Impact factor: 3.441

6.  Identification of regions of the chromosome of Neisseria meningitidis and Neisseria gonorrhoeae which are specific to the pathogenic Neisseria species.

Authors:  A Perrin; X Nassif; C Tinsley
Journal:  Infect Immun       Date:  1999-11       Impact factor: 3.441

7.  Intra-familial transmission and distribution of Prevotella intermedia and Prevotella nigrescens.

Authors:  T J van Steenbergen; C J Bosch-Tijhof; M D Petit; U Van der Velden
Journal:  J Periodontal Res       Date:  1997-05       Impact factor: 4.419

8.  Phylogeny of Bacteroides, Prevotella, and Porphyromonas spp. and related bacteria.

Authors:  B J Paster; F E Dewhirst; I Olsen; G J Fraser
Journal:  J Bacteriol       Date:  1994-02       Impact factor: 3.490

9.  PCR-based subtractive hybridization and differences in gene content among strains of Helicobacter pylori.

Authors:  N S Akopyants; A Fradkov; L Diatchenko; J E Hill; P D Siebert; S A Lukyanov; E D Sverdlov; D E Berg
Journal:  Proc Natl Acad Sci U S A       Date:  1998-10-27       Impact factor: 11.205

10.  The gene encoding a Prevotella loescheii lectin-like adhesin contains an interrupted sequence which causes a frameshift.

Authors:  J N Manch-Citron; J Allen; M Moos; J London
Journal:  J Bacteriol       Date:  1992-11       Impact factor: 3.490
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