Literature DB >> 1257055

Reaction of HeLa cell methyl-labelled 28S ribosomal RNA with sodium bisulphite: a conformational probe for methylated sequences.

J P Goddard, B E Maden.   

Abstract

The reaction of 14C methyl-labelled HeLa cell 28 S ribosomal RNA with sodium bisulphite was studied. Using conditions under which 30% of the total cytidine residues were de-aminated to uridine, the reactivities of individual cytidine residues near particular methylation sites differed widely; some underwent almost quantitative reaction, some showed intermediate reactivity and others were almost inert. The possible value of this method as a conformational probe for ribosomal RNA is discussed.

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Year:  1976        PMID： 1257055      PMCID： PMC342913          DOI： 10.1093/nar/3.2.431

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  13 in total

1.  Correlation between three-dimensional structure and chemical reactivity of transfer RNA.

Authors:  J D Robertus; J E Ladner; J T Finch; D Rhodes; R S Brown; B F Clark; A Klug
Journal:  Nucleic Acids Res       Date:  1974-07       Impact factor: 16.971

2.  Accessible and inaccessible bases in yeast phenylalanine transfer RNA as studied by chemical modification.

Authors:  D Rhodes
Journal:  J Mol Biol       Date:  1975-05-25       Impact factor: 5.469

3.  The determination of secondary structure in the poly(C) tract of encephalomyocarditis virus RNA with sodium bisulphite.

Authors:  J Goodchild; P Fellner; A G Porter
Journal:  Nucleic Acids Res       Date:  1975-06       Impact factor: 16.971

4.  Conversion of exposed cytidine residues to uridine residues in Escherichia coli formylmethionine transfer ribonucleic acid.

Authors:  J P Goddard; L H Schulman
Journal:  J Biol Chem       Date:  1972-06-25       Impact factor: 5.157

5.  The methylated nucleotide sequences in HELA cell ribosomal RNA and its precursors.

Authors:  B E Maden; M Salim
Journal:  J Mol Biol       Date:  1974-09-05       Impact factor: 5.469

6.  Nucleotide sequences from the low molecular weight ribosomal RNA of Escherichia coli.

Authors:  G G Brownlee; F Sanger
Journal:  J Mol Biol       Date:  1967-02-14       Impact factor: 5.469

7.  Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose.

Authors:  G G Brownlee; F Sanger
Journal:  Eur J Biochem       Date:  1969-12

8.  Base composition of ribosomal RNA precursors in the HeLa cell nucleolus: further evidence of non-conservative processing.

Authors:  M Willems; E Wagner; R Laing; S Penman
Journal:  J Mol Biol       Date:  1968-03-14       Impact factor: 5.469

9.  Reaction of sodium bisulfite with uracil, cytosine, and their derivatives.

Authors:  H Hayatsu; Y Wataya; K Kai; S Iida
Journal:  Biochemistry       Date:  1970-07-07       Impact factor: 3.162

10.  The effects of methionine deprivation on ribosome synthesis in HeLa cells.

Authors:  M H Vaughan; R Soeiro; J R Warner; J E Darnell
Journal:  Proc Natl Acad Sci U S A       Date:  1967-10       Impact factor: 11.205
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  6 in total

1.  Evidence on the conformation of HeLa-cell 5.8S ribosomal ribonucleic acid from the reaction of specific cytidine residues with sodium bisulphite.

Authors:  J M Kelly; J P Goddard; E H Maden
Journal:  Biochem J       Date:  1978-08-01       Impact factor: 3.857

2.  The kinetics of bisulphite modification of reactive residues in E. coli tRNA2Phe.

Authors:  M Lowdon; J P Goddard
Journal:  Nucleic Acids Res       Date:  1976-12       Impact factor: 16.971

3.  Studies on the primary and secondary structure of potato spindle tuber viroid: products of digestion with ribonuclease A and ribonuclease T1, and modification with bisulfite.

Authors:  H Domdey; P Jank; L Sänger; H J Gross
Journal:  Nucleic Acids Res       Date:  1978-04       Impact factor: 16.971

4.  Early hypomethylation of 2'-O-ribose moieties in hepatocyte cytoplasmic ribosomal RNA underlies the protein synthetic defect produced by CCl4.

Authors:  G A Clawson; J R MacDonald; C H Woo
Journal:  J Cell Biol       Date:  1987-08       Impact factor: 10.539

5.  Reassessment of Viroid RNA Cytosine Methylation Status at the Single Nucleotide Level.

Authors:  Francesco Di Serio; Enza Maria Torchetti; José-Antonio Daròs; Beatriz Navarro
Journal:  Viruses       Date:  2019-04-18       Impact factor: 5.048

6.  Multiple links between 5-methylcytosine content of mRNA and translation.

Authors:  Ulrike Schumann; He-Na Zhang; Tennille Sibbritt; Anyu Pan; Attila Horvath; Simon Gross; Susan J Clark; Li Yang; Thomas Preiss
Journal:  BMC Biol       Date:  2020-04-15       Impact factor: 7.431
  6 in total

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