Literature DB >> 167355

The determination of secondary structure in the poly(C) tract of encephalomyocarditis virus RNA with sodium bisulphite.

J Goodchild, P Fellner, A G Porter.   

Abstract

The degree of secondary structure in the poly(C) tract of encephalomyocarditis virus (EMCV) RNA has been investigated using sodium bisulphite, which brings about the hydrolysis of non-base-paired cytidylic acid to uridylic acid in RNA. The percentage conversion of C to U in the poly(C) region of native EMCV RNA was similar to that found in a synthetic polynucleotide lacking secondary structure [poly(C)]. When poly(I) was annealed to either native or denatured EMCV RNA, it protected the poly(C) tract from the action of bisulphite. It is concluded that the poly(C) tract of EMCV RNA in solution is very largely single-stranded.

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Year:  1975        PMID: 167355      PMCID: PMC343475          DOI: 10.1093/nar/2.6.887

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN: 0305-1048            Impact factor:   16.971


  11 in total

Review 1.  Animal RNA viruses: genome structure and function.

Authors:  A J Shatkin
Journal:  Annu Rev Biochem       Date:  1974       Impact factor: 23.643

2.  Inactivation of valine acceptor ativity by a C-U missense change in the anticodon of yeast valine transfer ribonucleic acid.

Authors:  R W Chambers; S Aoyagi; Y Furukawa; H Zawadzka; O S Bhanot
Journal:  J Biol Chem       Date:  1973-08-10       Impact factor: 5.157

3.  Nucleotide sequence of the gene coding for the bacteriophage MS2 coat protein.

Authors:  W Min Jou; G Haegeman; M Ysebaert; W Fiers
Journal:  Nature       Date:  1972-05-12       Impact factor: 49.962

4.  Presence of a large poly(rC) tract within the RNA of encephalomyocarditis virus.

Authors:  A Porter; N Carey; P Fellner
Journal:  Nature       Date:  1974-04-19       Impact factor: 49.962

5.  Conversion of exposed cytidine residues to uridine residues in Escherichia coli formylmethionine transfer ribonucleic acid.

Authors:  J P Goddard; L H Schulman
Journal:  J Biol Chem       Date:  1972-06-25       Impact factor: 5.157

6.  Poly(C) in animal viral RNAs.

Authors:  F Brown; J Newman; J Stott; A Porter; D Frisby; C Newton; N Carey; P Fellner
Journal:  Nature       Date:  1974-09-27       Impact factor: 49.962

7.  Conformation of polyribouridylic acid in solution.

Authors:  L D Inners; G Felsenfeld
Journal:  J Mol Biol       Date:  1970-06-14       Impact factor: 5.469

8.  The cleavage of DNA by the oxygen-dependent reaction of bisulfite.

Authors:  H Hayatsu; R C Miller
Journal:  Biochem Biophys Res Commun       Date:  1972-01-14       Impact factor: 3.575

9.  Optical properties of sixteen dinucleoside phosphates.

Authors:  M M Warshaw; I Tinoco
Journal:  J Mol Biol       Date:  1966-09       Impact factor: 5.469

10.  A study of the thermal stability of ribosomes and biologically active subribosomal particles.

Authors:  R A Cox; H Pratt; P Huvos; B Higginson; W Hirst
Journal:  Biochem J       Date:  1973-07       Impact factor: 3.857

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  4 in total

1.  Reaction of HeLa cell methyl-labelled 28S ribosomal RNA with sodium bisulphite: a conformational probe for methylated sequences.

Authors:  J P Goddard; B E Maden
Journal:  Nucleic Acids Res       Date:  1976-02       Impact factor: 16.971

2.  Multiple links between 5-methylcytosine content of mRNA and translation.

Authors:  Ulrike Schumann; He-Na Zhang; Tennille Sibbritt; Anyu Pan; Attila Horvath; Simon Gross; Susan J Clark; Li Yang; Thomas Preiss
Journal:  BMC Biol       Date:  2020-04-15       Impact factor: 7.431

Review 3.  The 5'-untranslated region of picornaviral genomes.

Authors:  V I Agol
Journal:  Adv Virus Res       Date:  1991       Impact factor: 9.937

Review 4.  The long-lasting enigma of polycytidine (polyC) tract.

Authors:  Velia Penza; Stephen J Russell; Autumn J Schulze
Journal:  PLoS Pathog       Date:  2021-08-04       Impact factor: 6.823

  4 in total

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