Literature DB >> 12530893

Click chemistry to construct fluorescent oligonucleotides for DNA sequencing.

Tae Seok Seo1, Zengmin Li, Hameer Ruparel, Jingyue Ju.   

Abstract

"Click chemistry" 1,3-dipolar cycloaddition between alkynyl 6-carboxyfluorescein (FAM) and azido-labeled single-stranded (ss) DNA was carried out under aqueous conditions to produce FAM-labeled ssDNA in quantitative yield. The FAM-labeled ssDNA was successfully used as a primer to produce DNA sequencing products with single-base resolution in a capillary electrophoresis DNA sequencer with laser-induced fluorescence detection.

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Year:  2003        PMID： 12530893     DOI： 10.1021/jo026615r

Source DB:  PubMed          Journal:  J Org Chem        ISSN： 0022-3263            Impact factor:   4.354


  22 in total

Review 1.  Click chemistry, a powerful tool for pharmaceutical sciences.

Authors:  Christopher D Hein; Xin-Ming Liu; Dong Wang
Journal:  Pharm Res       Date:  2008-05-29       Impact factor: 4.200

2.  Click-Engineered, Bioresponsive, and Versatile Particle-Protein-Dye System.

Authors:  Mary K Burdette; Ragini Jenkins; Yuriy P Bandera; Haley Jones; Isabell K Foulger; Ashley Dickey; Anna-Liisa Nieminen; Stephen H Foulger
Journal:  ACS Appl Bio Mater       Date:  2019-07-01

3.  Biocompatible artificial DNA linker that is read through by DNA polymerases and is functional in Escherichia coli.

Authors:  Afaf H El-Sagheer; A Pia Sanzone; Rachel Gao; Ali Tavassoli; Tom Brown
Journal:  Proc Natl Acad Sci U S A       Date:  2011-06-27       Impact factor: 11.205

4.  Chemoselective covalent coupling of oligonucleotide probes to self-assembled monolayers.

Authors:  Neal K Devaraj; Gregory P Miller; Wataru Ebina; Boyko Kakaradov; James P Collman; Eric T Kool; Christopher E D Chidsey
Journal:  J Am Chem Soc       Date:  2005-06-22       Impact factor: 15.419

5.  C2'-pyrene-functionalized triazole-linked DNA: universal DNA/RNA hybridization probes.

Authors:  Sujay P Sau; Patrick J Hrdlicka
Journal:  J Org Chem       Date:  2011-11-16       Impact factor: 4.354

6.  Vibrational stark effect probes for nucleic acids.

Authors:  Lisa N Silverman; Michael E Pitzer; Peter O Ankomah; Steven G Boxer; Edward E Fenlon
Journal:  J Phys Chem B       Date:  2007-09-18       Impact factor: 2.991

7.  Direct measurement of cathepsin B activity in the cytosol of apoptotic cells by an activity-based probe.

Authors:  Matthew R Pratt; Matthew D Sekedat; Kyle P Chiang; Tom W Muir
Journal:  Chem Biol       Date:  2009-09-25

8.  Design and synthesis of substituted (1-(benzyl)-1H-1,2,3-triazol-4-yl)(piperazin-1-yl)methanone conjugates: study on their apoptosis inducing ability and tubulin polymerization inhibition.

Authors:  Kesari Lakshmi Manasa; Sowjanya Thatikonda; Dilep Kumar Sigalapalli; Sowmya Vuppaladadium; Ganthala Parimala Devi; Chandraiah Godugu; Mallika Alvala; Narayana Nagesh; Bathini Nagendra Babu
Journal:  RSC Med Chem       Date:  2020-09-08

Review 9.  Growing applications of "click chemistry" for bioconjugation in contemporary biomedical research.

Authors:  Kido Nwe; Martin W Brechbiel
Journal:  Cancer Biother Radiopharm       Date:  2009-06       Impact factor: 3.099

10.  Synthesis of C-4 and C-7 triazole analogs of zanamivir as multivalent sialic acid containing scaffolds.

Authors:  Yan Lu; Jacquelyn Gervay-Hague
Journal:  Carbohydr Res       Date:  2007-06-09       Impact factor: 2.104
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