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Literature DB >> 12524530

Structural analysis of the yeast SWI/SNF chromatin remodeling complex.

Corey L Smith¹, Rachel Horowitz-Scherer, Joan F Flanagan, Christopher L Woodcock, Craig L Peterson.

Abstract

Elucidating the mechanism of ATP-dependent chromatin remodeling is one of the largest challenges in the field of gene regulation. One of the missing pieces in understanding this process is detailed structural information on the enzymes that catalyze the remodeling reactions. Here we use a combination of subunit radio-iodination and scanning transmission electron microscopy to determine the subunit stoichiometry and native molecular weight of the yeast SWI/SNF complex. We also report a three-dimensional reconstruction of yeast SWI/SNF derived from electron micrographs.

Entities: Chemical Gene Species

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Year: 2003 PMID： 12524530 DOI： 10.1038/nsb888

Source DB: PubMed Journal: Nat Struct Biol ISSN： 1072-8368

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Cited

77 in total

1. Using atomic force microscopy to study nucleosome remodeling on individual nucleosomal arrays in situ.

Authors: H Wang; R Bash; J G Yodh; G Hager; S M Lindsay; D Lohr
Journal: Biophys J Date: 2004-09 Impact factor: 4.033

2. Distinct subregions of Swi1 manifest striking differences in prion transmission and SWI/SNF function.

Authors: Zhiqiang Du; Emily T Crow; Hyun Seok Kang; Liming Li
Journal: Mol Cell Biol Date: 2010-08-02 Impact factor: 4.272

3. Regulating SWI/SNF subunit levels via protein-protein interactions and proteasomal degradation: BAF155 and BAF170 limit expression of BAF57.

Authors: Jianguang Chen; Trevor K Archer
Journal: Mol Cell Biol Date: 2005-10 Impact factor: 4.272

4. Terminal association of Rad54 protein with the Rad51-dsDNA filament.

Authors: Konstantin Kiianitsa; Jachen A Solinger; Wolf-Dietrich Heyer
Journal: Proc Natl Acad Sci U S A Date: 2006-06-19 Impact factor: 11.205

5. Conformational flexibility in the chromatin remodeler RSC observed by electron microscopy and the orthogonal tilt reconstruction method.

Authors: Andres E Leschziner; Anjanabha Saha; Jacqueline Wittmeyer; Yongli Zhang; Carlos Bustamante; Bradley R Cairns; Eva Nogales
Journal: Proc Natl Acad Sci U S A Date: 2007-03-13 Impact factor: 11.205

Structural analysis of the yeast SWI/SNF chromatin remodeling complex.

1. Using atomic force microscopy to study nucleosome remodeling on individual nucleosomal arrays in situ.

2. Distinct subregions of Swi1 manifest striking differences in prion transmission and SWI/SNF function.

3. Regulating SWI/SNF subunit levels via protein-protein interactions and proteasomal degradation: BAF155 and BAF170 limit expression of BAF57.

4. Terminal association of Rad54 protein with the Rad51-dsDNA filament.

5. Conformational flexibility in the chromatin remodeler RSC observed by electron microscopy and the orthogonal tilt reconstruction method.

Review 6. ATP-dependent chromatin remodeling enzymes: two heads are not better, just different.

Review 7. Mechanisms of action and regulation of ATP-dependent chromatin-remodelling complexes.

8. Activation of the ADE genes requires the chromatin remodeling complexes SAGA and SWI/SNF.

9. Solution AFM studies of human Swi-Snf and its interactions with MMTV DNA and chromatin.

10. Three-dimensional structure of human chromatin accessibility complex hCHRAC by electron microscopy.