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Literature DB >> 12517141

DNA detection and signal amplification via an engineered allosteric enzyme.

Alan Saghatelian¹, Kevin M Guckian, Desiree A Thayer, M Reza Ghadiri.

Abstract

Rapid, sensitive, and sequence-specific DNA detection can be achieved in one step using an engineered intrasterically regulated enzyme. The semi-synthetic inhibitor-DNA-enzyme (IDE) construct (left) rests in the inactive state but upon exposure to a complementary DNA sequence undergoes a DNA hybridization-triggered allosteric enzyme activation (right). The ensuing rapid substrate turnover provides the built-in signal amplification mechanism for detecting approximately 10 fmol DNA in less than 3 min under physiological conditions.

Entities: Gene

Mesh：

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Year: 2003 PMID： 12517141 PMCID： PMC2453066 DOI： 10.1021/ja027885u

Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419

17 in total

1. Active site-directed protein regulation.

Authors: B Kobe; B E Kemp
Journal: Nature Date: 1999-11-25 Impact factor: 49.962

2. In situ localization of PCR-amplified DNA and cDNA.

Authors: G J Nuovo
Journal: Methods Mol Biol Date: 2000

3. Electronic detection of nucleic acids: a versatile platform for molecular diagnostics.

Authors: R M Umek; S W Lin; J Vielmetter; R H Terbrueggen; B Irvine; C J Yu; J F Kayyem; H Yowanto; G F Blackburn; D H Farkas; Y P Chen
Journal: J Mol Diagn Date: 2001-05 Impact factor: 5.568

Review 4. Combining nucleic acid amplification and detection.

Authors: B Schweitzer; S Kingsmore
Journal: Curr Opin Biotechnol Date: 2001-02 Impact factor: 9.740

5. Scanometric DNA array detection with nanoparticle probes.

Authors: T A Taton; C A Mirkin; R L Letsinger
Journal: Science Date: 2000-09-08 Impact factor: 47.728

6. Spectral genotyping of human alleles.

Authors: L G Kostrikis; S Tyagi; M M Mhlanga; D D Ho; F R Kramer
Journal: Science Date: 1998-02-20 Impact factor: 47.728

7. Catalytic molecular beacons.

Authors: M N Stojanovic; P de Prada; D W Landry
Journal: Chembiochem Date: 2001-06-01 Impact factor: 3.164

8. Single-copy gene detection using branched DNA (bDNA) in situ hybridization.

Authors: A N Player; L P Shen; D Kenny; V P Antao; J A Kolberg
Journal: J Histochem Cytochem Date: 2001-05 Impact factor: 2.479

9. Determination of ancestral alleles for human single-nucleotide polymorphisms using high-density oligonucleotide arrays.

Authors: J G Hacia; J B Fan; O Ryder; L Jin; K Edgemon; G Ghandour; R A Mayer; B Sun; L Hsie; C M Robbins; L C Brody; D Wang; E S Lander; R Lipshutz; S P Fodor; F S Collins
Journal: Nat Genet Date: 1999-06 Impact factor: 38.330

10. Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles.

Authors: R Elghanian; J J Storhoff; R C Mucic; R L Letsinger; C A Mirkin
Journal: Science Date: 1997-08-22 Impact factor: 47.728

25 in total

DNA detection and signal amplification via an engineered allosteric enzyme.

1. Active site-directed protein regulation.

2. In situ localization of PCR-amplified DNA and cDNA.

3. Electronic detection of nucleic acids: a versatile platform for molecular diagnostics.

Review 4. Combining nucleic acid amplification and detection.

5. Scanometric DNA array detection with nanoparticle probes.

6. Spectral genotyping of human alleles.

7. Catalytic molecular beacons.

8. Single-copy gene detection using branched DNA (bDNA) in situ hybridization.

9. Determination of ancestral alleles for human single-nucleotide polymorphisms using high-density oligonucleotide arrays.

10. Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles.

1. Genetically encoded force sensors for measuring mechanical forces in proteins.

2. Foldamers with hybrid biological and synthetic sequences as selective DNA fluorescent probes.

3. Design of molecular logic devices based on a programmable DNA-regulated semisynthetic enzyme.

4. Elastic energy driven polymerization.

Review 5. Structure-switching biosensors: inspired by Nature.

6. Template-directed covalent conjugation of DNA to native antibodies, transferrin and other metal-binding proteins.

7. DNA-CNT nanowire networks for DNA detection.

8. Guanylate kinase, induced fit, and the allosteric spring probe.

9. Electrochemical DNA detection via exonuclease and target-catalyzed transformation of surface-bound probes.

10. Monovalent streptavidin that senses oligonucleotides.