| Literature DB >> 12482433 |
Anne Lauritsen1, Britta M Dahl, Otto Dahl, Birte Vester, Jesper Wengel.
Abstract
Synthesis of an oligonucleotide containing one methylphosphonate locked nucleic acid (LNA) thymine monomer using the phosphoramidite approach is described. The binding affinity of this 9-mer methylphosphonate LNA towards complementary DNA and RNA oligonucleotides was increased compared to the reference DNA, but decreased compared to the reference LNA. In the 9-mer sequence context studied, introduction of a single methylphosphonate LNA monomer, contrary to a single LNA monomer, efficiently inhibits 3'-exonucleolytic degradation.Entities:
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Year: 2003 PMID： 12482433 DOI： 10.1016/s0960-894x(02)00882-x
Source DB: PubMed Journal: Bioorg Med Chem Lett ISSN： 0960-894X Impact factor: 2.823