| Literature DB >> 12466290 |
Hitomi Yatsuki1, Keiichiro Joh, Ken Higashimoto, Hidenobu Soejima, Yuji Arai, Youdong Wang, Izuho Hatada, Yayoi Obata, Hiroko Morisaki, Zhongming Zhang, Tetsuji Nakagawachi, Yuji Satoh, Tsunehiro Mukai.
Abstract
Mouse chromosome 7F4/F5, where the imprinting domain is located, is syntenic to human 11p15.5, the locus for Beckwith-Wiedemann syndrome. The domain is thought to consist of the two subdomains Kip2 (p57(kip2))/Lit1 and Igf2/H19. Because DNA methylation is believed to be a key factor in genomic imprinting, we performed large-scale DNA methylation analysis to identify the cis-element crucial for the regulation of the Kip2/Lit1 subdomain. Ten CpG islands (CGIs) were found, and these were located at the promoter sites, upstream of genes, and within intergenic regions. Bisulphite sequencing revealed that CGIs 4, 5, 8, and 10 were differentially methylated regions (DMRs). CGIs 4, 5, and 10 were methylated paternally in somatic tissues but not in germ cells. CGI8 was methylated in oocyte and maternally in somatic tissues during development. Parental-specific DNase I hypersensitive sites (HSSs) were found near CGI8. These data indicate that CGI8, called DMR-Lit1, is not only the region for gametic methylation but might also be the imprinting control region (ICR) of the subdomain.Entities:
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Year: 2002 PMID： 12466290 PMCID： PMC187562 DOI： 10.1101/gr.110702
Source DB: PubMed Journal: Genome Res ISSN： 1088-9051 Impact factor: 9.043