Literature DB >> 12459907

Spectroscopic and kinetic characterization of active site mutants of Desulfovibrio fructosovoransNi-Fe hydrogenase.

Antonio L DeLacey1, Victor M Fernandez, Marc Rousset, Christine Cavazza, E Claude Hatchikian.   

Abstract

Site-directed mutagenesis of amino acid residues proximate to the active site of the Ni-Fe hydrogenase of Desulfovibrio fructosovorans has been done. The different mutants have been analyzed by FTIR spectroscopy and compared with wild type enzyme. The changes observed in the spectra confirm that hydrogen bonds between the CN(-) ligands of the active site's Fe atom and certain neighbor amino acid residues stabilize the active center within the protein matrix. However, kinetic analysis of the mutants indicates that none of the replaced residues have an important role in the catalytic mechanism of the hydrogenase.

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Year:  2002        PMID： 12459907     DOI： 10.1007/s00775-002-0397-4

Source DB:  PubMed          Journal:  J Biol Inorg Chem        ISSN： 0949-8257            Impact factor:   3.358


  12 in total

1.  FTIR spectroelectrochemical characterization of the Ni-Fe-Se hydrogenase from Desulfovibrio vulgaris Hildenborough.

Authors:  Antonio L De Lacey; Cristina Gutiérrez-Sánchez; Víctor M Fernández; Isabel Pacheco; Inês A C Pereira
Journal:  J Biol Inorg Chem       Date:  2008-08-13       Impact factor: 3.358

2.  Crystallographic studies of [NiFe]-hydrogenase mutants: towards consensus structures for the elusive unready oxidized states.

Authors:  Anne Volbeda; Lydie Martin; Elodie Barbier; Oscar Gutiérrez-Sanz; Antonio L De Lacey; Pierre-Pol Liebgott; Sébastien Dementin; Marc Rousset; Juan C Fontecilla-Camps
Journal:  J Biol Inorg Chem       Date:  2014-10-15       Impact factor: 3.358

3.  Structural differences between the ready and unready oxidized states of [NiFe] hydrogenases.

Authors:  Anne Volbeda; Lydie Martin; Christine Cavazza; Michaël Matho; Bart W Faber; Winfried Roseboom; Simon P J Albracht; Elsa Garcin; Marc Rousset; Juan C Fontecilla-Camps
Journal:  J Biol Inorg Chem       Date:  2005-04-01       Impact factor: 3.358

4.  Strongly Coupled Redox-Linked Conformational Switching at the Active Site of the Non-Heme Iron-Dependent Dioxygenase, TauD.

Authors:  Christopher W John; Greg M Swain; Robert P Hausinger; Denis A Proshlyakov
Journal:  J Phys Chem B       Date:  2019-09-06       Impact factor: 2.991

5.  Fourier Transform Infrared Spectrovoltammetry and Quantitative Modeling of Analytes in Kinetically Constrained Redox Mixtures.

Authors:  Christopher W John; Denis A Proshlyakov
Journal:  Anal Chem       Date:  2019-07-12       Impact factor: 6.986

6.  Probing intermediates in the activation cycle of [NiFe] hydrogenase by infrared spectroscopy: the Ni-SIr state and its light sensitivity.

Authors:  Maria-Eirini Pandelia; Hideaki Ogata; Leslie J Currell; Marco Flores; Wolfgang Lubitz
Journal:  J Biol Inorg Chem       Date:  2009-07-21       Impact factor: 3.358

7.  FTIR spectroelectrochemical study of the activation and inactivation processes of [NiFe] hydrogenases: effects of solvent isotope replacement and site-directed mutagenesis.

Authors:  Antonio L De Lacey; Alejandro Pardo; Víctor M Fernández; Sebastian Dementin; Geraldine Adryanczyk-Perrier; E Claude Hatchikian; Marc Rousset
Journal:  J Biol Inorg Chem       Date:  2004-06-03       Impact factor: 3.358

8.  Hydrogen activation by biomimetic [NiFe]-hydrogenase model containing protected cyanide cofactors.

Authors:  Brian C Manor; Thomas B Rauchfuss
Journal:  J Am Chem Soc       Date:  2013-07-30       Impact factor: 15.419

9.  Density functional study of the catalytic cycle of nickel-iron [NiFe] hydrogenases and the involvement of high-spin nickel(II).

Authors:  Alejandro Pardo; Antonio L De Lacey; Víctor M Fernández; Hua-Jun Fan; Yubo Fan; Michael B Hall
Journal:  J Biol Inorg Chem       Date:  2006-03-02       Impact factor: 3.358

10.  Mechanism of hydrogen activation by [NiFe] hydrogenases.

Authors:  Rhiannon M Evans; Emily J Brooke; Sara A M Wehlin; Elena Nomerotskaia; Frank Sargent; Stephen B Carr; Simon E V Phillips; Fraser A Armstrong
Journal:  Nat Chem Biol       Date:  2015-11-30       Impact factor: 15.040
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