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Literature DB >> 12431436

Group II introns: highly specific endonucleases with modular structures and diverse catalytic functions.

Olga Fedorova¹, Linhui Julie Su, Anna Marie Pyle.

Abstract

Group II introns are large catalytic RNAs with a remarkable repertoire of reactions. Here we present construct designs and protocols that were used to develop a set of kinetic frameworks for studying the structure and reaction mechanisms of group II introns and ribozymes derived from them. In addition, we discuss application of these systems to structure/function analysis of the ai5gamma group II intron.

Mesh：

Substances：
RNA, Catalytic

Year: 2002 PMID： 12431436 DOI： 10.1016/s1046-2023(02)00239-6

Source DB: PubMed Journal: Methods ISSN： 1046-2023 Impact factor: 3.608

Keyword Cloud
Cited

16 in total

Review 1. RNA folding in living cells.

Authors: Georgeta Zemora; Christina Waldsich
Journal: RNA Biol Date: 2010-11-01 Impact factor: 4.652

2. Linking the group II intron catalytic domains: tertiary contacts and structural features of domain 3.

Authors: Olga Fedorova; Anna Marie Pyle
Journal: EMBO J Date: 2005-10-27 Impact factor: 11.598

3. A general two-step strategy to synthesize lariat RNAs.

Authors: Yangming Wang; Scott K Silverman
Journal: RNA Date: 2005-12-22 Impact factor: 4.942

Review 4. The RNA world on ice: a new scenario for the emergence of RNA information.

Authors: Alexander V Vlassov; Sergei A Kazakov; Brian H Johnston; Laura F Landweber
Journal: J Mol Evol Date: 2005-07-13 Impact factor: 2.395

5. The linear form of a group II intron catalyzes efficient autocatalytic reverse splicing, establishing a potential for mobility.

Authors: Michael Roitzsch; Anna Marie Pyle
Journal: RNA Date: 2009-01-23 Impact factor: 4.942

Group II introns: highly specific endonucleases with modular structures and diverse catalytic functions.

Review 1. RNA folding in living cells.

2. Linking the group II intron catalytic domains: tertiary contacts and structural features of domain 3.

3. A general two-step strategy to synthesize lariat RNAs.

Review 4. The RNA world on ice: a new scenario for the emergence of RNA information.

5. The linear form of a group II intron catalyzes efficient autocatalytic reverse splicing, establishing a potential for mobility.

6. Native Purification and Analysis of Long RNAs.

7. The brace for a growing scaffold: Mss116 protein promotes RNA folding by stabilizing an early assembly intermediate.

8. The 2'-OH group at the group II intron terminus acts as a proton shuttle.

9. Divalent metal ions tune the self-splicing reaction of the yeast mitochondrial group II intron Sc.ai5gamma.

10. Extensive mis-splicing of a bi-partite plant mitochondrial group II intron.