Literature DB >> 12414523

Gene transfer of human hepatocyte growth factor into rat skin wounds mediated by liposomes coated with the sendai virus (hemagglutinating virus of Japan).

Kuniaki Nakanishi1, Maki Uenoyama, Naruya Tomita, Ryuichi Morishita, Yasufumi Kaneda, Toshio Ogihara, Kunio Matsumoto, Toshikazu Nakamura, Akie Maruta, Shigeo Matsuyama, Toshiaki Kawai, Takashi Aurues, Takuya Hayashi, Tomosumi Ikeda.   

Abstract

Hepatocyte growth factor (HGF) regulates cell growth, cell motility, and morphogenesis in various types of cells, including epithelial and endothelial cells, indicating that it probably promotes epithelial repair and neovascularization during wound healing. To better understand the effects of HGF on wound healing, we performed human HGF-gene transfer into skin wounds in rats. The rat HGF mRNA levels, and human and rat HGF protein concentrations in the wounds in HGF gene-transfer rats were significantly elevated at 3 days, 3 to 14 days, and 3 and 14 days after gene transfer, respectively. An expression of human HGF mRNA and protein was revealed in squamous cells in the epidermis, in endothelial cells and smooth muscle cells in blood vessels, and in fibroblasts in granulation tissues at 3, 7, and 14 days after gene transfer in HGF gene-transfer rats. The wound lesion area in HGF gene-transfer rats was significantly less than that in control rats from 3 to 7 days after gene transfer. The re-epithelialization rate, microvessel counts in granulation tissues, proliferating cell nuclear antigen index of fibroblasts in granulation tissues, and the proliferating cell nuclear antigen index in the epidermis of HGF gene-transfer rats were significantly increased at 3 and 7 days after gene transfer. Semiquantitative reverse transcriptase-polymerase chain reaction revealed that the expression levels of transforming growth factor-beta1 and Colalpha2(I) mRNAs in the wounds of HGF gene-transfer rats were significantly decreased at 7 and 14 days, respectively. The hydroxyproline concentration in the wound was significantly less in HGF gene-transfer rats than in control rats at 3 days after gene transfer. These results suggest that HGF gene transfer into a skin wound may aid re-epithelialization and neovascularization in the early phase of wound healing, and that HGF may play a role in modulating cutaneous wound healing.

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Year:  2002        PMID： 12414523      PMCID： PMC1850767          DOI： 10.1016/S0002-9440(10)64453-7

Source DB:  PubMed          Journal:  Am J Pathol        ISSN： 0002-9440            Impact factor:   4.307


  29 in total

1.  Control of scarring in adult wounds by neutralising antibody to transforming growth factor beta.

Authors:  M Shah; D M Foreman; M W Ferguson
Journal:  Lancet       Date:  1992-01-25       Impact factor: 79.321

Review 2.  Human gene therapy.

Authors:  W F Anderson
Journal:  Science       Date:  1992-05-08       Impact factor: 47.728

3.  Marked stimulation of growth and motility of human keratinocytes by hepatocyte growth factor.

Authors:  K Matsumoto; K Hashimoto; K Yoshikawa; T Nakamura
Journal:  Exp Cell Res       Date:  1991-09       Impact factor: 3.905

4.  Increased expression of DNA cointroduced with nuclear protein in adult rat liver.

Authors:  Y Kaneda; K Iwai; T Uchida
Journal:  Science       Date:  1989-01-20       Impact factor: 47.728

5.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

Authors:  P Chomczynski; N Sacchi
Journal:  Anal Biochem       Date:  1987-04       Impact factor: 3.365

6.  Molecular cloning and expression of human hepatocyte growth factor.

Authors:  T Nakamura; T Nishizawa; M Hagiya; T Seki; M Shimonishi; A Sugimura; K Tashiro; S Shimizu
Journal:  Nature       Date:  1989-11-23       Impact factor: 49.962

7.  Angiogenesis induced by hepatocyte growth factor in non-infarcted myocardium and infarcted myocardium: up-regulation of essential transcription factor for angiogenesis, ets.

Authors:  M Aoki; R Morishita; Y Taniyama; I Kida; A Moriguchi; K Matsumoto; T Nakamura; Y Kaneda; J Higaki; T Ogihara
Journal:  Gene Ther       Date:  2000-03       Impact factor: 5.250

8.  Scatter factor induces blood vessel formation in vivo.

Authors:  D S Grant; H K Kleinman; I D Goldberg; M M Bhargava; B J Nickoloff; J L Kinsella; P Polverini; E M Rosen
Journal:  Proc Natl Acad Sci U S A       Date:  1993-03-01       Impact factor: 11.205

Review 9.  Hepatocyte growth factor (HGF) as a tissue organizer for organogenesis and regeneration.

Authors:  K Matsumoto; T Nakamura
Journal:  Biochem Biophys Res Commun       Date:  1997-10-29       Impact factor: 3.575

10.  Direct in vivo gene introduction into rat kidney.

Authors:  N Tomita; J Higaki; R Morishita; K Kato; H Mikami; Y Kaneda; T Ogihara
Journal:  Biochem Biophys Res Commun       Date:  1992-07-15       Impact factor: 3.575
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  6 in total

Review 1.  Gene therapy and wound healing.

Authors:  Sabine A Eming; Thomas Krieg; Jeffrey M Davidson
Journal:  Clin Dermatol       Date:  2007 Jan-Feb       Impact factor: 3.541

2.  A selective COX-2 inhibitor suppresses chronic pancreatitis in an animal model (WBN/Kob rats): significant reduction of macrophage infiltration and fibrosis.

Authors:  T Reding; D Bimmler; A Perren; L-K Sun; F Fortunato; F Storni; R Graf
Journal:  Gut       Date:  2005-12-01       Impact factor: 23.059

3.  LAT1 expression in normal lung and in atypical adenomatous hyperplasia and adenocarcinoma of the lung.

Authors:  Kuniaki Nakanishi; Hirotaka Matsuo; Yoshikatsu Kanai; Hitoshi Endou; Sadayuki Hiroi; Susumu Tominaga; Makio Mukai; Eiji Ikeda; Yuichi Ozeki; Shinsuke Aida; Toshiaki Kawai
Journal:  Virchows Arch       Date:  2005-09-21       Impact factor: 4.064

4.  Nonviral retrograde gene transfer of human hepatocyte growth factor improves neuropathic pain-related phenomena in rats.

Authors:  Toyokazu Tsuchihara; Sho Ogata; Koichi Nemoto; Takatoshi Okabayashi; Kuniaki Nakanishi; Naoki Kato; Ryuichi Morishita; Yasufumi Kaneda; Maki Uenoyama; Shinya Suzuki; Masatoshi Amako; Toshiaki Kawai; Hiroshi Arino
Journal:  Mol Ther       Date:  2008-10-21       Impact factor: 11.454

Review 5.  Hepatocyte Growth Factor Isoforms in Tissue Repair, Cancer, and Fibrotic Remodeling.

Authors:  Ognoon Mungunsukh; Elizabeth A McCart; Regina M Day
Journal:  Biomedicines       Date:  2014-11-05

6.  HGF accelerates wound healing by promoting the dedifferentiation of epidermal cells through β1-integrin/ILK pathway.

Authors:  Jin-Feng Li; Hai-Feng Duan; Chu-Tse Wu; Da-Jin Zhang; Youping Deng; Hong-Lei Yin; Bing Han; Hui-Cui Gong; Hong-Wei Wang; Yun-Liang Wang
Journal:  Biomed Res Int       Date:  2014-01-15       Impact factor: 3.411
  6 in total

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