| Literature DB >> 12370427 |
Jie Zhou1, Nasrin Ashouian, Marc Delepine, Fumihiko Matsuda, Christophe Chevillard, Roy Riblet, Carl L Schildkraut, Barbara K Birshtein.
Abstract
The 3' Ig heavy chain locus (Igh) regulatory region is the most downstream known element of the murine Igh gene cluster. We report here that the nearest non-Igh genes-Crip, Crp2, and Mta1-are located approximately 70 kb further downstream and are beyond the end of the domain of Igh transcriptional regulation. We have localized an origin of replication in MEL cells to a 3-kb segment located between the 3' Igh regulatory region and Crip. Sequences downstream of this origin are replicated by forks that move in both directions. Sequences upstream of this origin (Igh-C, -D, and -J) are replicated in a single direction through a 500-kb segment in which no active bidirectional origins can be detected. We propose that this origin may lie at or near the end of the Igh regulation domain.Entities:
Mesh：
Substances：
Year: 2002 PMID： 12370427 PMCID： PMC129745 DOI： 10.1073/pnas.212392399
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205