Literature DB >> 12241080

DNA supercoiling by gyrase is linked to nucleoid compaction.

Rogier Stuger1, Conrad L Woldringh, Coen C van der Weijden, Norbert O E Vischer, Barbara M Bakker, Rob J M van Spanning, Jacky L Snoep, Hans V Westerhoff.   

Abstract

The genes of E. coli are located on a circular chromosome of 4.6 million basepairs. This 1.6 mm long molecule is compressed into a nucleoid to fit inside the 1-2 microm cell in a functional format. To examine the role of DNA supercoiling as nucleoid compaction force we modulated the activity of DNA gyrase by electronic, genetic, and chemical means. A model based on physical properties of DNA and other cell components predicts that relaxation of supercoiling expands the nucleoid. Nucleoid size did not increase after reduction of DNA gyrase activity by genetic or chemical means, but nucleoids did expand upon chemical inhibition of gyrase in chloramphenicol-treated cells, indicating that supercoiling may help to compress the genome.

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Year:  2002        PMID： 12241080     DOI： 10.1023/a:1020318705894

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  12 in total

1.  Extensive regulation compromises the extent to which DNA gyrase controls DNA supercoiling and growth rate of Escherichia coli.

Authors:  P R Jensen; C C Van Der Weijden; L B Jensen; H V Westerhoff; J L Snoep
Journal:  Eur J Biochem       Date:  1999-12

2.  H-NS mediated compaction of DNA visualised by atomic force microscopy.

Authors:  R T Dame; C Wyman; N Goosen
Journal:  Nucleic Acids Res       Date:  2000-09-15       Impact factor: 16.971

3.  The expression of the Escherichia coli fis gene is strongly dependent on the superhelical density of DNA.

Authors:  R Schneider; A Travers; G Muskhelishvili
Journal:  Mol Microbiol       Date:  2000-10       Impact factor: 3.501

Review 4.  The role of co-transcriptional translation and protein translocation (transertion) in bacterial chromosome segregation.

Authors:  Conrad L Woldringh
Journal:  Mol Microbiol       Date:  2002-07       Impact factor: 3.501

5.  Osmotic compaction of supercoiled DNA into a bacterial nucleoid.

Authors:  T Odijk
Journal:  Biophys Chem       Date:  1998-07-13       Impact factor: 2.352

Review 6.  Hypothesis: chromosome separation in Escherichia coli involves autocatalytic gene expression, transertion and membrane-domain formation.

Authors:  V Norris
Journal:  Mol Microbiol       Date:  1995-06       Impact factor: 3.501

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Authors:  P Dworsky
Journal:  Z Allg Mikrobiol       Date:  1974

8.  Chloramphenicol causes fusion of separated nucleoids in Escherichia coli K-12 cells and filaments.

Authors:  J M van Helvoort; J Kool; C L Woldringh
Journal:  J Bacteriol       Date:  1996-07       Impact factor: 3.490

9.  Polymer-mediated compaction and internal dynamics of isolated Escherichia coli nucleoids.

Authors:  S Cunha; C L Woldringh; T Odijk
Journal:  J Struct Biol       Date:  2001-10       Impact factor: 2.867

10.  Lethal overproduction of the Escherichia coli nucleoid protein H-NS: ultramicroscopic and molecular autopsy.

Authors:  R Spurio; M Dürrenberger; M Falconi; A La Teana; C L Pon; C O Gualerzi
Journal:  Mol Gen Genet       Date:  1992-01
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  20 in total

Review 1.  H-NS Regulates Gene Expression and Compacts the Nucleoid: Insights from Single-Molecule Experiments.

Authors:  Ricksen S Winardhi; Jie Yan; Linda J Kenney
Journal:  Biophys J       Date:  2015-10-06       Impact factor: 4.033

2.  Active transcription of rRNA operons condenses the nucleoid in Escherichia coli: examining the effect of transcription on nucleoid structure in the absence of transertion.

Authors:  Julio E Cabrera; Cedric Cagliero; Selwyn Quan; Catherine L Squires; Ding Jun Jin
Journal:  J Bacteriol       Date:  2009-04-24       Impact factor: 3.490

Review 3.  Integration of syntactic and semantic properties of the DNA code reveals chromosomes as thermodynamic machines converting energy into information.

Authors:  Georgi Muskhelishvili; Andrew Travers
Journal:  Cell Mol Life Sci       Date:  2013-06-15       Impact factor: 9.261

4.  Growth Phase-Dependent Chromosome Condensation and Heat-Stable Nucleoid-Structuring Protein Redistribution in Escherichia coli under Osmotic Stress.

Authors:  Nafiseh Rafiei; Martha Cordova; William Wiley Navarre; Joshua N Milstein
Journal:  J Bacteriol       Date:  2019-11-05       Impact factor: 3.490

5.  Bacterial Nucleoid: Interplay of DNA Demixing and Supercoiling.

Authors:  Marc Joyeux
Journal:  Biophys J       Date:  2019-09-26       Impact factor: 4.033

6.  Rv3852 (H-NS) of Mycobacterium tuberculosis Is Not Involved in Nucleoid Compaction and Virulence Regulation.

Authors:  Nina T Odermatt; Claudia Sala; Andrej Benjak; Gaëlle S Kolly; Anthony Vocat; Andréanne Lupien; Stewart T Cole
Journal:  J Bacteriol       Date:  2017-07-25       Impact factor: 3.490

7.  In vivo and in vitro patterns of the activity of simocyclinone D8, an angucyclinone antibiotic from Streptomyces antibioticus.

Authors:  Lisa M Oppegard; Bree L Hamann; Kathryn R Streck; Keith C Ellis; Hans-Peter Fiedler; Arkady B Khodursky; Hiroshi Hiasa
Journal:  Antimicrob Agents Chemother       Date:  2009-03-09       Impact factor: 5.191

8.  The effects of polydisperse crowders on the compaction of the Escherichia coli nucleoid.

Authors:  Da Yang; Jaana Männik; Scott T Retterer; Jaan Männik
Journal:  Mol Microbiol       Date:  2020-02-05       Impact factor: 3.501

9.  Spatial organization of RNA polymerase and its relationship with transcription in Escherichia coli.

Authors:  Xiaoli Weng; Christopher H Bohrer; Kelsey Bettridge; Arvin Cesar Lagda; Cedric Cagliero; Ding Jun Jin; Jie Xiao
Journal:  Proc Natl Acad Sci U S A       Date:  2019-09-16       Impact factor: 11.205

10.  Bacterial symbiont subpopulations have different roles in a deep-sea symbiosis.

Authors:  Tjorven Hinzke; Manuel Kleiner; Mareike Meister; Rabea Schlüter; Christian Hentschker; Jan Pané-Farré; Petra Hildebrandt; Horst Felbeck; Stefan M Sievert; Florian Bonn; Uwe Völker; Dörte Becher; Thomas Schweder; Stephanie Markert
Journal:  Elife       Date:  2021-01-06       Impact factor: 8.140
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