Literature DB >> 8763959

Chloramphenicol causes fusion of separated nucleoids in Escherichia coli K-12 cells and filaments.

J M van Helvoort1, J Kool, C L Woldringh.   

Abstract

Chloramphenicol is frequently used for better visualization of the Escherichia coli nucleoid. Here, we show that chloramphenicol causes not only rounding off of the nucleoid but also fusion of as many as four separated nucleoids. Nucleoid fusion occurred in fast-growing cells and in filaments obtained by dicF antisense RNA induction or in ftsZ84(Ts) and pbpB(Ts) mutants. Thus, treatment with chloramphenicol erroneously suggests that DNA segregation is inhibited.

Entities:  

Mesh：

Substances：

Year:  1996        PMID： 8763959      PMCID： PMC178188          DOI： 10.1128/jb.178.14.4289-4293.1996

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  18 in total

1.  Regulation of the expression of the cell-cycle gene ftsZ by DicF antisense RNA. Division does not require a fixed number of FtsZ molecules.

Authors:  F Tétart; J P Bouché
Journal:  Mol Microbiol       Date:  1992-03       Impact factor: 3.501

2.  Direct observation of fusion of bacterial nuclei.

Authors:  M SCHAECHTER; V O LAING
Journal:  J Bacteriol       Date:  1961-04       Impact factor: 3.490

3.  [Alterations in internal structure of Escherichia coli caused by antibiotics].

Authors:  B STEINBERG
Journal:  Schweiz Z Pathol Bakteriol       Date:  1952

4.  ftsZ is an essential cell division gene in Escherichia coli.

Authors:  K Dai; J Lutkenhaus
Journal:  J Bacteriol       Date:  1991-06       Impact factor: 3.490

5.  Division behavior and shape changes in isogenic ftsZ, ftsQ, ftsA, pbpB, and ftsE cell division mutants of Escherichia coli during temperature shift experiments.

Authors:  P E Taschner; P G Huls; E Pas; C L Woldringh
Journal:  J Bacteriol       Date:  1988-04       Impact factor: 3.490

6.  Involvement of FtsZ in coupling of nucleoid separation with septation.

Authors:  F Tétart; R Albigot; A Conter; E Mulder; J P Bouché
Journal:  Mol Microbiol       Date:  1992-03       Impact factor: 3.501

7.  Toporegulation of bacterial division according to the nucleoid occlusion model.

Authors:  C L Woldringh; E Mulder; P G Huls; N Vischer
Journal:  Res Microbiol       Date:  1991 Feb-Apr       Impact factor: 3.992

8.  FtsZ and nucleoid segregation during outgrowth of Bacillus subtilis spores.

Authors:  S R Partridge; R G Wake
Journal:  J Bacteriol       Date:  1995-05       Impact factor: 3.490

9.  Nucleoid partitioning in Escherichia coli during steady-state growth and upon recovery from chloramphenicol treatment.

Authors:  J M van Helvoort; C L Woldringh
Journal:  Mol Microbiol       Date:  1994-08       Impact factor: 3.501

10.  The initiation cascade for chromosome replication in wild-type and Dam methyltransferase deficient Escherichia coli cells.

Authors:  A Løbner-Olesen; F G Hansen; K V Rasmussen; B Martin; P L Kuempel
Journal:  EMBO J       Date:  1994-04-15       Impact factor: 11.598
View more
  22 in total

1.  Cell-Size Homeostasis and the Incremental Rule in a Bacterial Pathogen.

Authors:  Maxime Deforet; Dave van Ditmarsch; João B Xavier
Journal:  Biophys J       Date:  2015-08-04       Impact factor: 4.033

2.  Chromosome condensation in the absence of the non-SMC subunits of MukBEF.

Authors:  Qinhong Wang; Elena A Mordukhova; Andrea L Edwards; Valentin V Rybenkov
Journal:  J Bacteriol       Date:  2006-06       Impact factor: 3.490

3.  DNA supercoiling by gyrase is linked to nucleoid compaction.

Authors:  Rogier Stuger; Conrad L Woldringh; Coen C van der Weijden; Norbert O E Vischer; Barbara M Bakker; Rob J M van Spanning; Jacky L Snoep; Hans V Westerhoff
Journal:  Mol Biol Rep       Date:  2002       Impact factor: 2.316

4.  Active transcription of rRNA operons condenses the nucleoid in Escherichia coli: examining the effect of transcription on nucleoid structure in the absence of transertion.

Authors:  Julio E Cabrera; Cedric Cagliero; Selwyn Quan; Catherine L Squires; Ding Jun Jin
Journal:  J Bacteriol       Date:  2009-04-24       Impact factor: 3.490

5.  Lack of S-adenosylmethionine results in a cell division defect in Escherichia coli.

Authors:  E B Newman; L I Budman; E C Chan; R C Greene; R T Lin; C L Woldringh; R D'Ari
Journal:  J Bacteriol       Date:  1998-07       Impact factor: 3.490

6.  Effects of chromosome underreplication on cell division in Escherichia coli.

Authors:  E Botello; K Nordström
Journal:  J Bacteriol       Date:  1998-12       Impact factor: 3.490

7.  Time-dependent effects of transcription- and translation-halting drugs on the spatial distributions of the Escherichia coli chromosome and ribosomes.

Authors:  Somenath Bakshi; Heejun Choi; Jagannath Mondal; James C Weisshaar
Journal:  Mol Microbiol       Date:  2014-10-22       Impact factor: 3.501

8.  Robustness of the Process of Nucleoid Exclusion of Protein Aggregates in Escherichia coli.

Authors:  Ramakanth Neeli-Venkata; Antti Martikainen; Abhishekh Gupta; Nadia Gonçalves; Jose Fonseca; Andre S Ribeiro
Journal:  J Bacteriol       Date:  2016-01-04       Impact factor: 3.490

9.  Release of compact nucleoids with characteristic shapes from Escherichia coli.

Authors:  S B Zimmerman; L D Murphy
Journal:  J Bacteriol       Date:  2001-09       Impact factor: 3.490

10.  Effects of perturbing nucleoid structure on nucleoid occlusion-mediated toporegulation of FtsZ ring assembly.

Authors:  Qin Sun; William Margolin
Journal:  J Bacteriol       Date:  2004-06       Impact factor: 3.490
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.